- ￥490
- AMEKO
- 国产
- RC61022-500ml
- 2025年07月06日
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- 详细信息
- 文献和实验
- 技术资料
- 保存条件：
室温,12个月
- 库存：
现货
- 供应商：
联硕生物
- 规格：
500ml
注意事项：不能用于流式细胞分析。
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文献和实验complexes by pulse centrifugation (15 seconds at 3,000 RPM). Wash the bead/complexes 3 times in 500µl ice-cold TBS-mod buffer(comment 4); ;To elute the putative ChIP-Flag complexes (by competition with 3x Flag-peptide) add 100µl of TBS-mod buffer+3µl (5µg/µl
. 4. When finished, immerse membrane in Blocking buffer and block overnight. H. Antibodies and detection 1. Incubate with primary antibody diluted in Blocking buffer for 2 hours at room temp. 2. Wash 3 x 10 min with PBST. 1 x10min secondary washing
for 20 minutes in 500 ml bottles in the RC5-B using the GS3 rotor. Resuspend the cell pellets in old media and transfer to two bottles, centrifuge as before, and decant the media. The cell pellets can be frozen at -70degC at this point. 3. Resuspend
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