- ￥280
- 麦格
- 中国
- CE0051-250ml
- 2025年07月09日
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- 详细信息
- 文献和实验
- 技术资料
- 保存条件：
室温,12个月
- 保质期：
室温,12个月
- 英文名：
Michel's washing buffer
- 库存：
999
- 供应商：
麦飞生物
- 规格：
250ml
注意事项:不能用于流式细胞分析。
储运及效期:室温,12个月
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文献和实验CCMB buffer to make chemical competent cell
TOP10 chemically competent cells ( modified from http://openwetware.org/wiki/TOP10_chemically_competent_cells#CCMB80_buffer) I also use this protocol to make Ecoli compent cell. It is pretty good. You can start from the fresh clone on plate
the protein concentration of the extract and transfer the appropriate amount of your sample to a new tube. Aliquot and freeze the stock at -20℃ or below.3. Add Trident Sample Buffer to your sample and boil at 100℃ for 5 minutes to denature the proteins. Spin
. 4. When finished, immerse membrane in Blocking buffer and block overnight. H. Antibodies and detection 1. Incubate with primary antibody diluted in Blocking buffer for 2 hours at room temp. 2. Wash 3 x 10 min with PBST. 1 x10min secondary washing
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