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- Biolegend
- 423114
- 2025年07月15日
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- 详细信息
- 文献和实验
- 技术资料
- 库存：
大量
- 英文名：
Zombie Violet™ Fixable Viability Kit
- 保质期：
见COA
- 供应商：
维百奥生物
- 保存条件：
接收后，在 -20°C 下储存
维百奥生物库存现货，特价销售Zombie Violet™ Fixable Viability Kit！
| 产品信息
品名：Zombie Violet™ Fixable Viability Kit
别名：Fixable Dye, Fixable Viability Dye
品牌：Biolegend
货号：423114
规格：500 tests
| 产品描述
Calcein Red-AM is a fluorogenic, cell-permeant fluorescent probe that indicates cellular health by detecting the activity of nonspecific esterases. When the acetoxymethyl ester is intact the probe is non-fluorescent, but when it is cleaved by nonspecific esterases present in healthy cells, the probe can be excited and emit at 560 nm/574 nm respectively. The signal of Calcein Red-AM is proportional to the cell vitality, as esterase activity decreases in cells with poor vitality. This cell-permeant probe is not retained with fixation and permeabilization.
| 产品细节
1）Preparation
Zombie Violet™ Fixable Viability Kit is composed of lyophilized Zombie Violet™ dye and anhydrous DMSO. For reconstitution, bring the kit to room temperature; add 100 µl of DMSO to one vial of Zombie Violet™ dye until fully dissolved. 100 tests = 1 vial of Zombie Violet™ + DMSO, 500 tests = 5 vials of Zombie Violet™ + DMSO.
2）Storage & Handling
Store kit at -20°C upon receipt. Do not open vials until needed. Once the DMSO is added to the Zombie Violet™ dye, use immediately, or store at -20°C in a dry place and protected from light, preferably in a desiccator or in a container with desiccant for no more than one month.
3）Application
FC, ICFC - Quality tested；ICC - Verified
4）Recommended Usage
Each lot of this product is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometry, the suggested dilution is 1:100-1:1000 for 1-10 million cells. For immunocytochemistry, the suggested dilution is 1:1000. It is recommended that the reagent be titrated for optimal performance for each application, as optimal dosage varies with cell type.
5）Application Notes
Zombie Violet™ dye is excited by the Violet laser (405 nm) and has fluorescence emission maximum at 423 nm. If using in a multi-color panel design, filter optimization may be required depending on other fluorophores used. Zombie Violet™ dye has similar emission to Brilliant Violet 421™.
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文献和实验LIVE/DEAD® Violet Viability/Vitality Kit
Protect from light When stored as directed, kit components are stable for at least 6 months * Calcein violet AM may hydrolyze if exposed to moisture. Number
Vybrant® DyeCycle Violet Stain
Stability Vybrant® DyeCycle™ Violet stain 200 μL 5 mM solution in deionized water 2-6?C
. 2. Combining AbC™ anti-Rat/Hamster and ArC™ Amine Reactive Compensation Bead Kits The ArC™ Amine Reactive Compensation Bead Kit (Cat. no. A10346) is designed to facilitate compensation when using any of the LIVE/DEAD® fixable dead cell stains
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