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- 保存条件:
负20摄氏度
- 保质期:
3个月
- 英文名:
pCMV-EGFP-DNMT3A-Neo Plasmid
- 库存:
100
- 供应商:
LSM BIO
- 规格:
2ug
pCMV-EGFP-DNMT3A-Neo Plasmid
Catalog No.: PVT69150
pCMV-EGFP-DNMT3A-Neo Plasmid
Alias:NM_175629.2;DNMT3A2; HESJAS; M.HsaIIIA; TBRS
Host: Mammalian cells
Use(s): Protein expression
Fragment Type: ORF
Fragmented species: Human
Prokaryotic resistance: Amp
Screening Markers: Neo/G418
Fluorescence labeling: Green
Promoter: CMV
Competent cells: DH5a
Temperature: 37Celsius
Caution:
1. This product is FOR RESEARCH USE ONLY!
2. The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3. Shipping temperature is 2-8 degrees centigrade.
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文献和实验就可以了。 如果我找到个质粒,将这个基因连上去之后,怎么才能鉴别/筛选目的基因正反向的问题 你可以根据质粒图谱看,定向将启动子CMV后 将基因定向克隆进去,利用酶切反应及连接的特异性。 ps: 感觉你对自己的实验目的都不是很清楚,这很危险!宁可不做,就怕费力不讨好! bigbang_0_0 用pEGFP质粒,很适合GFP标签的蛋白的表达; 如果做稳定转染,需要用G418筛选标记 (neo r)
表达His和sumo标签蛋白 pET28a+EGFP 大肠杆菌质粒 Kan 绿色荧光原核载体,0.1mM TPTG,20度诱导过夜 pET28a+DsRed2 大肠杆菌质粒 Kan 红色荧光原核载体,0.1mM TPTG,20度诱导过夜 pET28a+EYFP
initiation site (6) to further increase the translation efficiency in eukaryotic cells. The MCS in pEGFP-N2 is between the immediate early promoter of CMV (PCMV IE ) and the EGFP coding sequences. Genes cloned into the MCS will be expressed as fusions
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