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- 保质期：
１年
- 英文名：
Frozen-EZ Yeast Transformation II Kit
- 库存：
大量
- 供应商：
简石生物
- 保存条件：
－４度
HIGHLIGHTS
- Fast: Yeast cells with high transformation efficiencies can be prepared in under 10 minutes.
- Simple: Easy method to transform with single or multiple plasmids in ≤ 1 hour with carrier DNA.
- Versatile: Can be used with S. cerevisiae, as well as other fungi, including C. albicans, S. pombe, and P. pastoris. Compatible with both circular and linear DNA.
DESCRIPTION
The Frozen-EZ Yeast Transformation II Kit is designed to make yeast transformations and library screening easier and more efficient than currently available methods. The yeast cells prepared with the Frozen-EZ Yeast Transformation II Kit can be used immediately for transformation or can be stored (i.e., ≤ -70°C) for use at a later time. Yeast prepared with this kit can be transformed with both circular and linear DNAs. Also, the Frozen-EZ Yeast Transformation II Kit can be used with other fungi including C. albicans, S. pombe, and P. pastors.
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文献和实验Yeast Transformation and the Preparation of Frozen Spheroplasts for Electroporation
Transformation methods for the baker’s yeast Saccharomyces cerevisiae have allowed for rapid advances in molecular genetic studies with this model microorganism, for the development of alternative expression systems to prepare recombinant
This Protocol allows You to prepare Frozen Yeast cells that are competent for transformation after thawing After Dohmen et al. (1991) Yeast 7: 691-692. See Schiestl et al (1993) The ability to make yeast cells
High Efficiency Yeast Transformation (Gietz Long Protocol)
the cells to complete at least two divisions.ii) Transformation efficiency (transformants/ µg plasmid/108 cells) remains constant for 3 to 4 celldivisions.4. Harvest the culture in a sterile 50 ml centrifuge tube at 3000 x g (5000 rpm) for 5 min.5. Pour
