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Description:Recombinant protein of human TAR (HIV-1) RNA binding protein 2 (TARBP2), transcript variant 3, full length, with N-terminal HIS tag, expressed in E.Coli, 50ug
Accn:NM_004178
Unipro ID:Q15633
Synonyms:LOQS; TRBP; TRBP1; TRBP2
Species:Human
Amount:50 ug
Delivery time:现货
Expression sequence:A DNA sequence encoding human full-length TARBP2
Tags:N-His
PredictedMW:39
Buffer:25 mM Tris-HCl, pH 8.0, 150 mM NaCl, 1% sarkosyl, 10% glycerol
Stability:Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
Bioactivity
Purity:> 80% as determined by SDS-PAGE and Coomassie blue staining
Concentration:>0.05 µg/µL as determined by microplate BCA method
Preparation
Endotoxin
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Background:HIV-1, the causative agent of acquired immunodeficiency syndrome (AIDS), contains an RNA genome that produces a chromosomally integrated DNA during the replicative cycle. Activation of HIV-1 gene expression by the transactivator Tat is dependent on an RNA regulatory element (TAR) located downstream of the transcription initiation site. The protein encoded by this gene binds between the bulge and the loop of the HIV-1 TAR RNA regulatory element and activates HIV-1 gene expression in synergy with the viral Tat protein. Alternative splicing results in multiple transcript variants encoding different isoforms. This gene also has a pseudogene. [provided by RefSeq, Jul 2008]
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文献和实验RNA transcripts are produced from the 5′ long terminal repeat (LTR) of human immunodeficiency virus type-1 (HIV-1) under the complex control of viral regulatory sequences ( 1 – 7 ). The LTR from a prototypical
【申请上传】Current Protocols in Immunology
.5 Detection Assays for HIV Proteins Unit 12.6 Detection of HIV DNA and RNA Using PCR Unit 12.7 Regulation of the HIV Promoter/Enhancer Unit 12.8 In Situ Hybridization for Detection of HIV RNA Unit 12.9 In Vitro Evaluation of Experimental Agents for Anti
. et al. (2003) Antennapedia and HIV transactivator of transcription (TAT) ‘protein transduction domains’ promote endocytosis of high molecular weight cargo upon binding to cell surface glycosaminoglycans. J. Biol. Chem. 278, 35109–35114 [13] Fischer
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