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- 文献和实验
- 技术资料
- 英文名:
1,3-Dicyclopropyl-thiourea
- 供应商:
北京孚博
- CAS号:
59814-70-9
- 规格:
1g
CAS:59814-70-9
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文献和实验pieces and frozen at 2757C until needed. Homogenization was performed using a Polytron PT-1200C (Kinematica, Luzern, Switzerland) directly in the lysis buffer (8.4 M urea, 2.4 M thiourea, 5% CHAPS, 50 mM DTT and 1% IPG buffer 3–10 (total volume 2 mL/g fat
for this purpose. ::Chaotrophe: urea and thiourea::Reductant: DTT, Tris(2-carboxyethyl) phosphine (TCEP), tributyl phosphine (TBP)::Detergent: CHAPS, triton x-100::Ampholytes::Protease inhibitors Some researchers also add tris buffer.Chaotrophe.Urea
buffers we use are listed below, obviously there are other reductants and detergents that can be used for this purpose.:: Chaotrophe: urea and thiourea:: Reductant: DTT, Tris(2-carboxyethyl) phosphine (TCEP), tributyl phosphine (TBP):: Detergent
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