Working anti-feline猫 IgG (Fc)-

ELISA 原理与分类

效应。假使在被测分子的不同位点上含有多个相同的决定簇，例如HBsAg的a决定簇，也可用针对此决定簇的同一单抗分别包被固相和制备酶结合物。但在HBsAg的检测中应注意亚型问题，HBsAg有adr、adw、ayr、ayw4个亚型，虽然每种亚型均有相同的a决定簇的反应性，这也是用单抗作夹心法应注意的问题。双抗体夹心法测抗原的另一注意点是类风湿因子（RF）的干扰。RF是一种自身抗体，多为IgM型，能和多种动物IgG的Fc段结合。用作双抗体夹心法检测的血清标本中如含有RF，它可充当抗原成份，同时与固相抗体和酶标

Isotype-specific immunostaining with mouse primary antibodies on mouse tissue

  Staining mouse tissue with anti-mouse Ig (H&L chain) secondary reagents inevitabily causes staining of interstitial immunoglobulins, B cells, plasma cells and macrophages with Ig bound to Fc

Preparation of Screen-Printed Electrochemical Immunosensors for Estradiol, and Their Application in Biological Fluids

(rabbit anti-mouse IgG, then monoclonal mouse anti-E2 ) are immobilized by passive adsorption onto the working electrode surface. A competitive immunoassay is then performed using an alkaline-phosphatase-labeled E2 conjugate. Electrochemical measurements