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文献和实验, 5% [v/v] polyethylene glycol, 100 µM aurintricarboxylic acid) were annealed at 37°C for 12 hours. Unreacted probe was subsequently removed by washing. The slides were coated with nuclear track emulsion (Kodak NTB-2), developed after exposures at 4℃
The In Situ PCR:Amplification and Detection in a Cellular Context
retention of DNA during subsequent denaturation, hybridization and washing (4). After denaturation in 95% formamide, 0.1xSSC for 15 minutes at 65°C, the slides were cooled to 4°C in 0.1xSSC, dipped in water, and dehydrated in graded alcohols. Virus-specific
The Mouse as Model System to Study Host‐Pathogen Interactions in Influenza A Infections
variants of standard laboratory virus strains ) stock solution in PBS Phosphate‐buffered saline (PBS; Invitrogen) 8‐ to 12
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