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- 详细信息
- 文献和实验
- 技术资料
- 库存:
99
- 英文名:
pCMV-Pdk1(mouse)-3×FLAG-Neo
- 保质期:
3个月~12个月
- 供应商:
上海禾午生物有限公司
- 保存条件:
负20℃&负80℃
- 规格:
干粉&甘油菌
| 名称 | pCMV-Pdk1(mouse)-3×FLAG-Neo |
| 别称 | NM_172665.5;B830012B01; D530020C15Rik |
| 基因长 | 1305bp |
| 质粒宿主 | 哺乳细胞 |
| 质粒用途 | 蛋白表达 |
| 片段类型 | ORF |
| 片段物种 | 小鼠 |
| 原核抗性 | Amp |
| 筛选标记 | Neo/G418 |
| 启动子 | CMV |
| 复制子 | pUC |
| 感受态 | DH5a |
| 温度 | 37度 |
| 背景载体 | pCMV-T7-MCS-3×FLAG-WPRE-Neo |
| 正向引物 | CMV-F |




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| pCMV-Insr(rat)-3×FLAG-Neo |
| pCMV-EGFP-STX6(human)-Neo |
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文献和实验activity in vitro, e.g., small compounds can activate the phosphoinositide-dependent protein kinase 1 (PDK1). Compounds binding to an allosteric site on a protein kinase may produce conformational changes at the ATP-binding site within the active site
Blastocyst embryo transfer into pseudopregnant recipient female mouse(图)
and exhibit bad planes of anesthesia when heavy. This is also a very inexpensive mouse to use. As an alternate, another strain I have used with considerable success is B6D2F1. These mice are hardy and display hybrid vigor. The Equipment For surgery
PROTOCOL General anesthesia inject i.p. 250µl/mouse of the ANESTHETIC mixture for verify the anesthetic effect check plantar reflexes (animal must be unresponsive to squeezing hind feet) Preparation
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