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- 2025年07月15日
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文献和实验), Nucleic Acid Electrophoresis (Springer Lab Manual). Heidelberg: Springer. pp 1–53. 2.Thermo Fisher Scientific Inc (2010) Nucleic Acid Detection and Analysis. In: Molecular Probes Handbook: A Guide to Fluorescent Probes and Labeling Technologies. pp 349–360
Combined 3C-ChIP-Cloning (6C) Assay: A Tool to Unravel Protein-Mediated Genome Architecture
type of interest, grown under appropriate cell culture conditions with appropriate medium Cells, bacterial, competent, high-efficiency ( 5 x 109 cfu/µg DNA) XL10-Gold ultracompetent cells (Stratagene) have produced optimal results.
citrate phosphate dextrose and dilute the solution 1:1 with Hank's buffered salt solution (without CaCl2 , MgCl2 , MgSO4 ) and layer on equal volumes of Histopaque to create well-defined layers. 4) Centrifuge (30 min, 740 g, low break
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