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- 详细信息
- 文献和实验
- 技术资料
- 供应商:
上海阿拉丁生化科技股份有限公司
- 库存:
现货
- 英文名:
Recombinant RPS20 Antibody
- 保存条件:
-20°C储存,避免反复冻融;超低温运输
- 规格:
Ab125924-10μl/Ab125924-100μl/Ab125924-1ml/Ab125924-50μl
| 规格: | Ab125924-10μl | 产品价格: | ¥499.90 |
|---|---|---|---|
| 规格: | Ab125924-100μl | 产品价格: | ¥2099.90 |
| 规格: | Ab125924-1ml | 产品价格: | ¥16999.90 |
| 规格: | Ab125924-50μl | 产品价格: | ¥1299.90 |
基本信息
产品名称:Recombinant RPS20 Antibody
规格或纯度:ExactAb™, Validated, 重组, 2.0 mg/mL
应用:WB, IF/ICC, IHC
特异性:RPS20
种属反应性:人(Human), 小鼠(Mouse), 大鼠(Rat), 仓鼠(Hamster)
免疫原:A synthetic peptide derived from human RPS20 (AA 56-70)
阳性对照:WB: HeLa, Ramos, A431, Hep G2, RAW 264.7, NIH/3T3 and C6 cell lysates. IHC: Human tonsil tissue. IF/ICC: HeLa cells.
偶联:Unconjugated
产品属性
克隆类型:重组抗体
Format:Whole IgG
亚型:Rabbit IgG
SDS-PAGE:150 kDa
纯化方法:Antigen affinity purified
物理外观:Liquid
储存缓冲液:50mM Tris-Glycine, 150mM NaCl, 40% Glycerol, 0.05% BSA, 0.01% Sodium azide, pH 7.4
防腐剂:0.01% Sodium azide
浓度:2.0 mg/mL
储存条件:-20°C储存,避免反复冻融
运输条件:超低温运输
稳定性与储存:4°C 短期储存(1-2 周)。-20°C下长期保存(24个月)。收货后建议分装。避免冷冻/解冻循环。
功能和特点
宿主种属:兔(Rabbit)
种属反应性:人(Human), 小鼠(Mouse), 大鼠(Rat), 仓鼠(Hamster)
偶联:Unconjugated
图片
Recombinant RPS20 Antibody (Ab125924) - Western Blot
All lanes: Recombinant RPS20 Antibody (Ab125924) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution
Predicted band size: 13 kDa
Observed band size: 15 kDa
Exposure time: 3.0 s
Recombinant RPS20 Antibody (Ab125924) - IHC
Immunohistochemistry analysis of paraffin-embedded Human tonsil using Recombinant RPS20 Antibody (Ab125924). High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.
Recombinant RPS20 Antibody (Ab125924) - IF/ICC
IF analysis of Recombinant RPS20 Antibody (Ab125924) in HeLa cells. The cells were fixed and permeabilized with 100% methanol for 5 minutes, and blocked with 5% BSA for 1 hour at room temperature. Cells were stained with Recombinant RPS20 Antibody (Ab125924) at 1/200 dilution in blocking buffer overnight at 4°C, and then incubated with Goat Anti-Rabbit IgG H&L (AF647) (Ab181958) at a dilution of 1/1000 for 1 hour at room temperature in the dark (red). Cells were counterstained with DAPI (blue).
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Differentiating Neural Stem Cells into Neurons and Glial Cells
of the secondary antibody host species, 1% BSA, 0.1% Triton®-X in D-PBS with Ca2 and Mg2 ). Note: If you are using a surface antigen such as GalC, omit Triton®-X from the blocking buffer. b. Remove the blocking buffer and incubate the cells overnight
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