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- 详细信息
- 文献和实验
- 技术资料
- 供应商:
上海阿拉丁生化科技股份有限公司
- 库存:
现货
- 英文名:
SIRT6 Antibody
- 保存条件:
-20°C储存,避免反复冻融;超低温运输
- 规格:
Ab127697-100μl/Ab127697-1ml/Ab127697-50μl/Ab127697-10μl
| 规格: | Ab127697-100μl | 产品价格: | ¥2099.90 |
|---|---|---|---|
| 规格: | Ab127697-1ml | 产品价格: | ¥16999.90 |
| 规格: | Ab127697-50μl | 产品价格: | ¥1299.90 |
| 规格: | Ab127697-10μl | 产品价格: | ¥499.90 |
基本信息
产品名称:Recombinant SIRT6 Antibody
规格或纯度:ExactAb™, Validated, Carrier Free, Recombinant, 0.6 mg/mL
应用:WB, IF/ICC
特异性:SIRT6
种属反应性:人(Human), 小鼠(Mouse), 大鼠(Rat)
免疫原:A synthetic peptide derived from human SIRT6 (AA 19-33)
阳性对照:WB: K562, HeLa, MCF7, NIH/3T3 and C6 cell lysates. IF/ICC: HeLa cells.
偶联:Unconjugated
产品属性
克隆类型:重组抗体
Format:Whole IgG
亚型:Rabbit IgG
轻链亚型:kappa
SDS-PAGE:150 kDa
纯化方法:Protein A purified
物理外观:Liquid
储存缓冲液:10mM PBS, 0.09% Sodium Azide, pH 7.4
防腐剂:0.01% Sodium azide
浓度:0.6 mg/mL
储存条件:-20°C储存,避免反复冻融
运输条件:超低温运输
稳定性与储存:4°C 短期储存(1-2 周)。-20°C下长期保存(24个月)。收货后建议分装。避免冷冻/解冻循环。
功能和特点
宿主种属:兔(Rabbit)
种属反应性:人(Human), 小鼠(Mouse), 大鼠(Rat)
偶联:Unconjugated
图片
Recombinant SIRT6 Antibody (Ab127697) - Western Blot
All lanes: Recombinant SIRT6 Antibody (Ab127697) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution
Predicted band size: 36 kDa
Observed band size: 37, 41 kDa
Exposure time: 60.0 s
Recombinant SIRT6 Antibody (Ab127697) - Western Blot
All lanes: Recombinant SIRT6 Antibody (Ab127697) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution
Predicted band size: 36 kDa
Observed band size: 42 kDa
Exposure time: 20.0 s
Recombinant SIRT6 Antibody (Ab127697) - IF/ICC
IF analysis of Recombinant SIRT6 Antibody (Ab127697) in HeLa cells. The cells were fixed with 4% PFA for 10 minutes, permeabilized with 0.3% Triton X-100 for 10 minutes and then blocked with 5% BSA for 1 hour at room temperature. Cells were stained with Recombinant SIRT6 Antibody (Ab127697) at 1/500 dilution in blocking buffer overnight at 4°C, and then incubated with Goat Anti-Rabbit IgG H&L (AF647) (Ab181958) at a dilution of 1/1000 for 1 hour at room temperature in the dark (red). Cells were counterstained with DAPI (blue).
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