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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
-20°C储存
- 英文名:
Phosphatase, Alkaline from Escherichia coli
- 库存:
期货
- 供应商:
上海阿拉丁生化科技股份有限公司
- 规格:
P755408-25U/P755408-100U
| 规格: | P755408-25U | 产品价格: | ¥2982.9 |
|---|---|---|---|
| 规格: | P755408-100U | 产品价格: | ¥8713.9 |
来自 大肠杆菌 的碱性磷酸酶已被用于: 荧光测定法中测定其在去磷酸化酶混合物中的酶活性 用于处理神经和 HL60 细胞裂解物中的 β-羟基 β-甲基戊二酰辅酶 A (HMG-CoA) 还原酶活性碱性磷酸酶用于偶联抗体和其他蛋白,用于 ELISA、免疫印迹和组织化学检测。需要高灵敏度时可用于蛋白标记。产品 P4069 以甘油缓冲水溶液形式提供。
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文献和实验4-9 except phosphate, avoid calcium chelators, add 50% glycerol for storage as liquid at -20C. Aliquot avoid repeated freeze/thaw cycles. Special care must be taken with deoxyribonuclease. Mix gently to avoid shear denaturation. In general, Dnase I is activated
Single tube confirmation PCR protocol
(200mg/l). - Incubate at 30°C for two to three days. *This step reduces background by eliminating aborted transformants. 2. Zymolyase treatment - Transfer a small portion of a well-isolated colony (~1 mm) into 50 µl solution
Preparation of End-Labeled DNA Probes by Conventional Kinase for DNA Footprinting Analysis
Approximately 100 μl of radiolabeled DNA 15 μl of 10X Restriction Enzyme Buffer 15 μl of 10 mM DTT 1.5 μl of 20 mg/ml BSA 20 Units of Restriction Enzyme ddH2 O to give an approximate final volume of 150 μl
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