碱性磷酸酶 来源于大肠杆菌，9001-78-9，buffer
ed aqueous glycerol solution, 20-50 units/mg protein (in glycine buffer)，阿拉丁- ￥2982.90 - 8713.90
- 阿拉丁
- 上海
- P755408
- 2026年01月06日
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- 详细信息
- 文献和实验
- 技术资料
- 保存条件：
-20°C储存
- 英文名：
Phosphatase, Alkaline from Escherichia coli
- 库存：
期货
- 供应商：
上海阿拉丁生化科技股份有限公司
- 规格：
P755408-25U/P755408-100U
| 规格： | P755408-25U | 产品价格： | ￥2982.9 |
|---|---|---|---|
| 规格： | P755408-100U | 产品价格： | ￥8713.9 |
英文名：Phosphatase, Alkaline from Escherichia coli
纯度或规格：buffered aqueous glycerol solution, 20-50 units/mg protein (in glycine buffer)
SPU：P755408
CAS：9001-78-9
存储温度：-20°C储存
运输条件：超低温运输
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文献和实验Single tube confirmation PCR protocol
(200mg/l). - Incubate at 30°C for two to three days. *This step reduces background by eliminating aborted transformants. 2. Zymolyase treatment - Transfer a small portion of a well-isolated colony (~1 mm) into 50 µl solution
Preparation of End-Labeled DNA Probes by Conventional Kinase for DNA Footprinting Analysis
Approximately 100 μl of radiolabeled DNA 15 μl of 10X Restriction Enzyme Buffer 15 μl of 10 mM DTT 1.5 μl of 20 mg/ml BSA 20 Units of Restriction Enzyme ddH2 O to give an approximate final volume of 150 μl
at 4°CEthidium bromide solution (20 mg/ml in H2O) (for duplex PCR only; see Steps 40-53)Forward and reverse primers (100 µM stock solutions in H2O)For duplex PCR (Steps 40-53), primers should be designed in order to obtain comparable amplifications
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