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- 文献和实验
- 技术资料
- 保存条件:
-20°C储存
- 英文名:
Phosphatase, Alkaline from porcine kidney
- 库存:
期货
- 供应商:
上海阿拉丁生化科技股份有限公司
- 规格:
P755396-100U
英文名:Phosphatase, Alkaline from porcine kidney
纯度或规格:lyophilized powder,≥100 DEA units/mg protein
SPU:P755396
CAS:9001-78-9
存储温度:-20°C储存
运输条件:超低温运输
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文献和实验Material 1. Source: rabbit skeletal muscle 2. Equipment: • mincer • glass-Teflon-homogenizer with pestle • sephacryl S-300 column (5 x 100 cm) • electrophoretic gels • centrifuge • sterile cheesecloth
Preparing a Selenomethionyl Protein
antibiotics (1000x conc.) 20mL amino acid 50x Stock (If precipitate is seen, heat to 60-70°C and shake.) Inoculate M9 with 50mL overnight culture and grow until an OD600 =0.5-0.6. (~2.0 - 2.5 hours) Add 100mg threonine, lysine hydrochloride
Protein purification: tropomyosin
4. (take supernatant) cool to +4oC; add 20 mg (NH4)2SO4 per 100 ml; stir for 20 min, centrifuge at 16,000 x g for 30 min 5. (take supernatant) add 30 gram (NH4)2SO4 per 100 ml; stir for 20 min; centrifuge at 16,000 x g for 30 min 6. (take pellet
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