- ￥6057.90
- 阿拉丁
- 上海
- L755436
- 2026年01月06日
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- 详细信息
- 文献和实验
- 技术资料
- 保存条件：
2-8°C储存
- 英文名：
Lumi-Light<sup>PLUS</sup> Western Blotting Substrate
- 库存：
期货
- 供应商：
上海阿拉丁生化科技股份有限公司
- 规格：
L755436-100ml
英文名：Lumi-LightPLUS Western Blotting Substrate
纯度或规格：pkg of 100 mL (1,000 cm2 membrane)
SPU：L755436
CAS：-
存储温度：2-8°C储存
运输条件：低温运输
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文献和实验Myosin Light Chain Preparation
Buffer A.Homogenizing Buffer: 28 liters: 50 mM KCl,20 mM Tris (pH 8.0),15 mM MCE, 0.2 mM MgAc2 (350 ml 4 M KCl,280 ml 2 M Tris,28 ml 15 M MCE,1.20 g MgAc2) Buffer B.Triton X-100 Buffer: 4 liters of Buffer A plus 40 ml X-100 (1% Triton final) Buffer C
Purification of Myosin Light Chain Kinase
layer ~5 ml of Buffer C on top of the resin,connect the column top and wash overnight at 45 ml/hr with Buffer G [Abs = 1.0,chart speed 0.5 mm/min,pump = 35x,6.6 min/tube (5 ml fractions),4℃].Under these conditions in the morning,recorder pen
DETECTION OF ß-GALACTOSIDASE AND ALKALINE PHOSPHATASE ACTIVITIES IN TISSUE
immediately before using. Stocks X-Phos (100X):10mg/ml 5-bromo-4-chloro-3-indolyl-phosphate (also referred to as BCIP) in H20. Store in the dark as aliquots at -200 C. Can be frozen and thawed several times. NBT (50X):50 mg/ml nitro
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