INT/BCIP 储备液，solution, pkg of

Alternate Sporulation Method for BY4743

supplement solution 2 mls 1 x his supplement 200 x his supplement solution 1 ml 1 x leu supplement 200 x leu supplement solution

原创：western protocol

mM TrisCl 6.057g Chromogenic solution 33ulNBT solution 33ulBCIP solution 10ml alkaline phosphatese buffer NBT solution:0.1g NBT in 10ml 70% N,N-dimethylformamide BCIP solution:0.5g BCIP in 10ml N,N-dimethylformamide Alkaline Phosphatese buffer 1L

Immunostaining with Anti-Digoxigenin Alkaline Phosphatase

, in Buffer 1. Buffer 2: 100  mM Tris-HCl pH 9.5, 50 mM MgCl2 , 100 mM NaCl (use pH 10 for blue color) Buffer 3: Buffer 2 with 0.5 mg/mL levamisole (make fresh) Color Detection Solution: 0.45 uL/mL NBT, 3.5 uL/mL BCIP, in Buffer 3.  (Make fresh)