Coelenterazine fcp，123437-33-2

荧光素酶和腔肠素Coelenterazine的选择及使用

素和O2参与。 Gussia荧光素酶：来源于海洋桡脚类生物Gaussia princeps，是一个19.9kDa单体酶，只有185个氨基酸，具有一个16aa 的分泌信号肽，可以被细胞分泌到细胞外，反应只需要腔肠素和O2参与。 腔肠素/Coelenterazine 美国Goldbio品牌的两种腔肠素，分别是天然腔肠素/Coelenterazine及其衍生物Coelenterazine 400a。 天然腔肠素/Coelenterazine：普 遍认为是天然的腔肠荧

Video-Rate Bioluminescence Imaging of Protein Secretion from a Living Cell

reaction of secreted GLase (luciferase) and coelenterazine (luciferin) are detected with an electron-multiplying charge-coupled device camera. An exocytotic event of protein secretion can be visualized using the protein fused to GLase with a time resolution

Study of GPCRProtein Interactions by BRET

resulting from the rapid oxidation of a cell-permeable coelenterazine substrate by the donor will transfer to the acceptor, which in turn fluoresces at a longer characteristic wavelength. Therefore, the occurrence of such energy transfer implies