TANKPE030默克密理博Millipore 30 LIT

SSR GEL and Silver Staining Protocol

to produce a 1 bp ladder. PGEM is loaded in a well separate from poly A. We do not use poly A for fingerprinting gels. Using a 144-well, microtiter 4X offset comb, load 3 ul of the mix: 3.4 ul 1X Perkin Elmer II PCR buffer 8.6 ul of 30 ng / ul PGEM 12 ul

Native Chromatin Preparation and Illumina/Solexa Library Construction

of PCR primer PE 1.0 (2X diluted with 1X TE, pH 7.4) 1 µL of PCR primer PE 2.0 (2X diluted with 1X TE, pH 7.4) ii. Denature at 98°C for 30 sec, followed by 18 amplification cycles (98°C, 10 sec; 65°C

SSR GEL and Silver Staining Protocol

for 2 min., ice and 5 μl 1X PE II buffer 2.5 μl of 400 ng / μl of sonicated Poly-dA 7.5 μl SSRP bufferVI. ELECTROPHORESIS:Note: Adding sodium acetate to the bottom reservoir during electrophoresis (Sheen and Seed, 1988, Biotechniques 6:942-944) produces