B501005-0005 生工 DEPC处理水5L Sang

RNase and DEPC Treatment: Fact or Laboratory Myth

(0.1% and 1%). It would be impossible to predict the different interactions of DEPC with all molecular biology reagents. The most cautious approach for making RNase-free solutions would be to mix molecular biology grade powdered reagents up in DEPC-treated water. Alternatively

Phenol/Freeze RNA Prep

% EtOH. Ppt. at -20 deg C for 1 hr. 13. Pellet, and wash pellet with 70% EtOH. 14. Resuspend with DEPC treated water (~30 µl). Store at -70 deg C.  

Primer Extension

). Add DEPC treated water to make up to 9µl (4.5µl if using 10pmoles/µl primer). Add 1µl T4 polynucleotide kinase from same kit. Incubate for 30 minutes at 37°C. Remove top and bottom cap from NAP-5 column (Amersham-Pharmacia Biotech