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- 详细信息
- 文献和实验
- 技术资料
- 保存条件：
ROOM TEMPERATURE
- 供应商：
西宝生物
- 英文名：
DEPC-TREATED WATER
- 库存：
200 ml
- 规格：
MOLECULAR BIOLOGY
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文献和实验How to make DEPC-treated water and Tris Buffer
Add 0.1 ml DEPC to 100 ml of the solution to be treated and shake vigorously to bring the DEPC into solution.Let the solution incubate for 12 hours at 37℃.Autoclave for 15 minutes to remove any trace of DEPC.Notes:How to make DEPC-treated Tris
Add 0.1% diethylpyrocarbonate (DEPC) to water, mix overnight, and then autoclave 20 min to destroy DEPC
, use DEPC-treated water to generate the solution. Although H2 O prepared with reverse osmosis systems like Milli-Q is free of RNases (Huang 1995) , it can be contaminated by microbial growth if the machine and piping are not well maintained
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