TS-18817赛默飞REACTI-BLOCK T-1 9孔

用全血直接分选 CD3+ T 细胞

- 3) 及 CD45 -PE (HI30，白细胞标志) 抗体染色后，进行流式分析，死细胞以 DAPI 染色先行排除，左右分别为分选前后的细胞群分布。   总结： 几次实验的结果，使用 Fab-TACS® Gravity column 来从全血直接进行 CD3+ T 细胞分选，平均得率为 84.5%，纯度大于 90%，细胞存活率高于 95%，且目标细胞不含标记，贴近天然，使下游实验更精确。

The OP9-DL1 System: Generation of T-Lymphocytes from Embryonic or Hematopoietic Stem Cells In Vitro

. Freeze the cells at -80°C, and then transfer them to liquid nitrogen for storage. Protocol 2: In Vitro Generation of T-Lymphocytes from ESCs Thawing ESCs 16. Prepare a 15-mL tube containing 12 mL of ESC medium. 17

T-cell dependent antigen specific in situ staining (Pe)

6- Wash twice in TBS-T. 7- counterstain with rabbit anti B-Pe 1:2000 (0.5 µg/ml Cortex Biochem). You may add 1% mouse serum to the anti-Pe antibodies, unless you plan to use mouse monoclonal further (see below). 8- wash twice in TBS-T. 9- block