Sino Biological C5AR1/C5R1/CD88重组蛋白 Nanodisc平台 Flag-Strep&His标签

C5AR1/C5R1/CD88重组蛋白|Recombinant Human C5AR1/C5R1/CD88 Protein (Nanodisc, Flag-Strep & His Tag)（产品说明）
蛋白名称：Human C5AR1/C5R1/CD88 Protein (Nanodisc, Flag-Strep & His Tag, thermostabilizing mutations)
蛋白构建：A DNA sequence encoding the Human C5AR1 (P21730)(Met1-Lys333, thermostabilizing mutations) was expressed and linked by a Fusion Protein, with a Flag and a polyhistidine tag at the N-terminus. Within the expressed regions, there are several proprietary thermostabilizing mutations.This protein was prepared using our Nanodisc platform. Nanodisc is a versatile tool for studying membrane proteins. Using styrene-maleic acid (SMA) copolymer, membrane proteins can be extracted directly from prokaryotic and eukaryotic expression systems in the absence of detergents to preserve the protein structure and function better. Compared to membrane scaffold proteins (MSPs) nanodiscs, SMA nanodiscs also have the advantage of preserving proteins' nature by maintaining native lipids surrounded without introducing any heterologous proteins, which allows studies of protein structure and functions in a native-like environment.
表达宿主：HEK293 Cells
蛋白纯度：≥ 85 % as determined by SDS-PAGE.
蛋白活性：1.Immobilized Recombinant Human C5AR1/C5R1/CD88 Protein (Nanodisc, Flag-Strep & His Tag) (Cat:12154-H91H-NA) at 5 μg/mL (100 μL/well) can bind Avdoralimab-Derived Anti-C5AR1 Antibody,Human IgG1, the EC50 is 4-15 ng/mL. 2.Immobilized Recombinant Human C5AR1/C5R1/CD88 Protein (Nanodisc, Flag-Strep & His Tag) (Cat:12154-H91H-NA) at 5 μg/mL (100 μL/well) can bind TJ210/MOR210-Derived Anti-C5AR1 Antibody,Human IgG1, the EC50 is 1-5 ng/mL.
蛋白内毒素：< 1.0 EU per μg protein as determined by the LAL method.
蛋白分子量：The recombinant Human C5AR1 comprises 366 amino acids and has a predicted molecular mass of 41.26 kDa. It migrates as an approximately 45 kDa band in SDS-PAGE under reducing conditions.
蛋白NP号：P21730
蛋白氨基酸序列：Met1-Lys333