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Recombinant Human PLAG1 Protein (N-His Tag), 重组人PLAG1蛋白 (N端His标签)
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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1mM EDTA, 4% Trehalose, 1% Mannitol.
- 保质期:
1 year
- 英文名:
Recombinant Human COL17A1 Protein, N-His
- 库存:
999
- 供应商:
abinScience
- 规格:
100ug
| Expression system | Mammalian Cells |
| Accession | P08667 |
| Protein length | Met1-Tyr459 |
| Alternative Names | Glycoprotein, G |
| Form | Lyophilized |
| Predicted molecular weight | 52.96 kDa |
| Applications | ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress |
| Storage buffer | Lyophilized from a solution in PBS pH 7.4, 1mM EDTA, 4% Trehalose, 1% Mannitol. |
| Purity | >90% as determined by SDS-PAGE. |
| Nature | Recombi\t |
| Species | (strain Pasteur vaccins / PV) (RABV) |
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文献和实验.3 ). Concatamers of tags are cloned and sequenced to yield a STAGE library. Tags in the library represent DNA fragments that were occupied by the DNA?binding protein, and mapping these tag sequences to the genome identifies the binding loci of the DNA?binding
Binding Protein,MBP)的大肠杆菌malE基因,其下游的多克隆位点便于目的基因插入,表达N端带有MBP的融合蛋白。此系统的pMAL载体除了含有氨苄霉素抗性,还含有LacZα基因,目的基因的插入导致其失活,通过X-gal平板可进行蓝白斑筛选。pMAL载体都含有一段编码蛋白酶识别位点的序列,融合蛋白纯化后,通过Factor Xa(X),Enterokinase (E)或 Genenase™ I (G)可将目的蛋白与MBP切割分离。如果不想在切割后留下任何外源氨基酸可以在设计引物时引入XmnⅠ
of complex and protein alone with the naked eye or a light microscope, crystals of covalently Cy5?labeled DNA can be directly used for X?ray diffraction data collection. Curr. Protoc. Nucleic Acid Chem. 46:7.15.1?7.15.8. © 2011 by John Wiley & Sons
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