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- 详细信息
- 文献和实验
- 技术资料
- 浓度:
Recommended Dilutions:Western Blotting (WB): 1:1,000-1:5,000 Flow Cytometry (FCM): 1:2,000 Immunocytochemistry (IC): 1:100-1:1,000
- 应用范围:
Western Blotting (WB), Flow Cytometry (FCM), Immunocytochemistry (IC)
- 宿主:
兔
- 适应物种:
Human, Mouse, Rat
- 保质期:
One year
- 克隆性:
Rabbit mAb
- 保存条件:
Store at -20 °C for one year. Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
- 形态:
液体
- 亚型:
Rabbit IgG
- 免疫原:
A synthesized peptide derived from human Histone H3.3
- 规格:
20ul/50ul/100ul
| 规格: | 20ul | 产品价格: | ¥620.0 |
|---|---|---|---|
| 规格: | 50ul | 产品价格: | ¥1200.0 |
| 规格: | 100ul | 产品价格: | ¥2200.0 |
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文献和实验Immunofluorescence: Rabbit Anti-Murine RELMα
for 25 minutes. 3. Incubate the tissue section with blocking buffer for 20 minutes. 4. Incubate the tissue section overnight at 4?C with Rabbit Anti-Murine RELMα at 4.0 ng/mL in 1X PBS with 0.01% Triton-X and 0.5% BSA. Wash the slide
Detection of Histone H3 Phosphorylation in Cultured Cells and Tissue Sections by Immunostaining
. The protocol described here allows the detection of phosphorylated histones in tissue-cultured cells and tissue sections by fluorescent or bright-field immunostaining analysis. Here we used a serine 10 specific P-histone H3 antibody to determine
FACS-Based Detection of Phosphorylated Histone H3 for the Quantitation of Mitotic Cells
scanner (FACS) is described, based on the presence of an intranuclear antigen present only in mitotic cells, detected using a specific, commercially available antibody. Cell staining and FACS analysis can be done in a single day, making this a rapid
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