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- 详细信息
- 文献和实验
- 技术资料
- 库存:
1000
- 英文名:
pCMV-Gosr1(mouse)-3×HA-Neo
- 保质期:
3年
- 供应商:
百欧博伟
- 保存条件:
-80
平台编号:bio-108196
名称: pCMV-Gosr1(mouse)-3×HA-Neo
别称: NM_016810.4;GOS-28; GOSRI; GS28
基因长: 750bp
规格: 少量干粉质粒(现货) 大提液体质粒(一周)
片段类型: ORF
荧光标记:
启动子: CMV
复制子: pUC
拷贝数:
感受态: DH5a
温度: 37度
背景载体:
正向引物: pCMV-MCS-3×HA-Neo
反向引物: CMV-F
诱导方式:
质粒简介
pCMV-Gosr1(mouse)-3×HA-Neo
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文献和实验Blastocyst embryo transfer into pseudopregnant recipient female mouse(图)
and exhibit bad planes of anesthesia when heavy. This is also a very inexpensive mouse to use. As an alternate, another strain I have used with considerable success is B6D2F1. These mice are hardy and display hybrid vigor. The Equipment For surgery
PROTOCOL General anesthesia inject i.p. 250µl/mouse of the ANESTHETIC mixture for verify the anesthetic effect check plantar reflexes (animal must be unresponsive to squeezing hind feet) Preparation
Vasectomized males are needed to engender pseudopregnancy in mature female mice. Such pseudopregnant females act as hormonally and physiologically competent recipients for microinjected fertilized one-cell eggs (see Chapter 20 ). Vasectomized
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