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- 详细信息
- 文献和实验
- 技术资料
- 库存:
1000
- 英文名:
pMT-V5-HisA
- 保质期:
3年
- 供应商:
百欧博伟
- 保存条件:
-80
平台编号:bio-109262
原核抗性: Amp
克隆菌株: DH5a
培养条件: 37度
质粒属性
片段类型:
片段物种:
原核抗性: Amp
真核抗性:
荧光标记:
pMT/V5-His is a 3.5 kb expression vector designed for use with the Drosophila Inducible Expression System. Upon transfection, the vector allows transient, inducible expression of your protein of interest in Drosophila cells. When cotransfected with the selection vectors, pCoHygro or pCoBlast, included with the appropriate DES Inducible Kit, pMT/V5-His allows selection of stable cell lines exhibiting inducible expression of the protein of interest.
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文献和实验2)、哺乳动物/真核表达载体 pcDNA3.1+ 不含任何标签的真核表达载体 amp/neo 5.4kb E. coli/mammals pcDNA3.1- 不含任何标签的真核表达载体 amp/neo 5.4kb E. coli/mammals pcDNA4/V5-His A, amp 含His标签的真核表达载体 amp/neo pcDNA 3.1(-) myc-hisA 含His标签的真核表达载体 amp/neo pcDNA 3.1(-) myc-hisB 含His标签
FINGERPRINTING PROTOCOL (AGAROSE GEL)
the same stock of dye. Store diluted stains in a foil covered container at 4℃. After staining, image gels using Fluorimager with the following scan settings: pixel size: 200 µm; digital resolution: 16 bits; detection sensitivity: high; PMT voltage: 950 V; filter
DNA fingerprinting - agarose gel
stains in a foil covered container at 4℃. After staining, image gels using Fluorimager with the following scan settings: pixel size: 200 µm; digital resolution: 16 bits; detection sensitivity: high; PMT voltage: 950 V; filter :530 nm. Crop gel images
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