- ￥3870 - 8940
- Sino Biological
- 北京
- 90975-C49H-B
- 2025年08月12日
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- 详细信息
- 文献和实验
- 技术资料
- 保存条件：
-20℃ to -80℃
- 保质期：
12个月
- 英文名：
Recombinant Cynomolgus CD25/IL-2R alpha Protein(ECD, His & AVI Tag),Biotinylated
- 库存：
99
- 供应商：
北京义翘神州科技股份有限公司
- 规格：
20.00 µg/100.00 µg
| 规格： | 20.00 µg | 产品价格： | ￥3870.0 |
|---|---|---|---|
| 规格： | 100.00 µg | 产品价格： | ￥8940.0 |
蛋白名称：Cynomolgus CD25 / IL-2R alpha Protein (ECD, His & AVI Tag), Biotinylated
蛋白构建：A DNA sequence encoding the Cynomolgus IL2RA (H6WS54) (Met1-Arg213) was expressed with a C-terminal polyhistidine tag followed by an AVI tag. The expressed protein was biotinylated in vivo by the Biotin-Protein ligase (BirA enzyme) which is co-expressed.
表达宿主：HEK293 Cells
蛋白纯度：≥ 95 % as determined by SDS-PAGE. ≥ 90 % as determined by SEC-HPLC.
蛋白活性：Immobilized Recombinant Rat CD25 / IL-2R alpha Protein (ECD, AVI & His Tag), Biotinylated (Cat: 90975-C49H-B ) at 2 μg/mL (100 μl/well) on streptavidin precoated (2 μg/mL, 100 μL/well) can bind Recombinant Human IL2 Protein, Low Endotoxin (Cat: 11848-HNAH1-E), the EC50 is 35-120 ng/mL.
蛋白内毒素：< 1.0 EU per μg protein as determined by the LAL method.
预测N端：Glu 22
蛋白分子量：The recombinant Cynomolgus IL2RA comprises 218 amino acids and has a predicted molecular mass of 24.9 kDa. It migrates as approximately 44.4 kDa band in SDS-PAGE under reducing conditions.
蛋白NP号：H6WS54
蛋白氨基酸序列：Met1-Arg213
蛋白标签：C-His-AVI
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文献和实验after specifically interacting with its receptors. Human IL-2 is a 133-amino acid polypeptide with a molecular mass of 15-18 kDa. IL-2 signaling is mediated by a multichain receptor complex consisting of an alpha (CD25), beta (CD122), and gamma (CD132) chain. The IL-2R
Intracellular Cytokine Staining Protocol
-gamma, TNF-alpha, IL-2, and IL-4, a combination of PMA (a PKC activator) and Ionomycin (a calcium ionophore) or anti-CD3 antibodies are used. To induce IL-6, IL-10 or TNF-alpha production by monocytes, stimulation of PBMC's with LPS is used.Note: Optimal
Intracellular Cytokine Staining Protocol
to produce IFN-gamma, TNF-alpha, IL-2, and IL-4, a combination of PMA (a PKC activator) and Ionomycin (a calcium ionophore) or anti-CD3 antibodies are used. To induce IL-6, IL-10 or TNF-alpha production by monocytes, stimulation of PBMC's with LPS is used
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