小鼠 JAM-2/JAM-B蛋白|JAM-2/JAM-B protein|JAM-2/JAM-B(Mouse, C-human IgG1-Fc)(产品说明)
蛋白名称:JAM-2/JAM-B蛋白, JAM-2/JAM-B protein
蛋白构建:A DNA sequence encoding the mouse JAM-2 (NP_076333.3) extracellular domain (Met 1-Asn 236) was fused with the Fc region of human IgG1 at the C-terminus.
表达宿主:HEK293 Cells
蛋白纯度:> 70 % as determined by SDS-PAGE
蛋白活性:Measured by the ability of the immobilized protein to support the adhesion of Jurkat human leukemic T cells . When 8 x 10E4 cells/well are added to JAM2-coated plates (0.2 μg/ml and 100 μl/well), approximately 40-55% will adhere specifically after 60 minutes at 37℃.
蛋白内毒素:< 1.0 EU per μg of the protein as determined by the LAL method
预测N端:Phe 29
蛋白分子量:The secreted recombinant mouse JAM-2/Fc is a disulfide-linked homodimeric protein. The reduced monomer comprises 449 amino acids and has a predicted molecular mass of 50.3 kDa. rm JAM-2/Fc monomer migrates as an approximately 65 kDa band in SDS-PAGE under reducing conditions.
蛋白NP号:NP_076333.3
蛋白氨基酸序列:Met1-Asn236
蛋白标签:C-human IgG1-Fc
蛋白保存条件:Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.