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T25
C6/C6细胞系/C6细胞株/C6大鼠神经胶质瘤细胞
Cell line name C6
Synonyms C-6; C 6; RGC-6; RGC6; RGc6
Accession CVCL_0194
Secondary accession CVCL_4060
Resource Identification Initiative To cite this cell line use: C6 (RRID:CVCL_0194)
Comments Doubling time: 14.75 hours (PubMed=2303324); 24-28 hours (DOI=10.5005/jp-journals-10082-02230); ~24 hours (CLS=500142); ~25-30 hours (DSMZ=ACC-550).
Transformant: ChEBI; CHEBI:50102; N-methyl-N-nitrosourea (NMU).
Omics: Deep quantitative proteome analysis.
Omics: Mitochondrial genome sequenced.
Derived from site: In situ; Brain; UBERON=UBERON_0000955.
Disease Rat malignant glioma (NCIt: C64069)
Species of origin Rattus norvegicus (Rat) (NCBI Taxonomy: 10116)
Breed/subspecies: Wistar Furth; RS=RS:0000982.
Hierarchy Children:
CVCL_ZB60 (C6 rho-0#14) CVCL_1970 (C6-BU-1) CVCL_6336 (C6-SF2)
CVCL_4V35 (C6/ACNU) CVCL_C9FG (C6/Cas9-hyg) CVCL_5657 (C6/lacZ)
CVCL_B7TS (C6rho0) CVCL_5H90 (C6TK-)
Sex of cell Male
Age at sampling Age unspecified
Category Cancer cell line
Publications
CLPUB00233
Lightbody J.
Establishment of differentiated clonal strains of glial brain cells in culture.
Fed. Proc. 27:720-720(1968)
PubMed=4873531; DOI=10.1126/science.161.3839.370
Benda P., Lightbody J., Sato G.H., Levine L., Sweet W.H.
Differentiated rat glial cell strain in tissue culture.
Science 161:370-371(1968)
DOI=10.1007/BF02618370
Stulberg C.S., Coriell L.L., Kniazeff A.J., Shannon J.E.
The animal cell culture collection.
In Vitro 5:1-16(1970)
PubMed=3468959
Yoshida T., Shimizu K., Ushio Y., Mogami H., Sakamoto Y.
Possibility of overcoming of ACNU resistance in an ACNU-resistant subline of C6 rat glioma.
No To Shinkei 38:1065-1070(1986)
PubMed=2303323
Brauner T., Schmid A., Hulser D.F.
Tumor cell invasion and gap junctional communication. I. Normal and malignant cells confronted in monolayer cultures.
Invasion Metastasis 10:18-30(1990)
PubMed=2303324
Brauner T., Hulser D.F.
Tumor cell invasion and gap junctional communication. II. Normal and malignant cells confronted in multicell spheroids.
Invasion Metastasis 10:31-48(1990)
PubMed=19381449; DOI=10.1007/s11060-009-9875-7; PMCID=PMC2730996
Barth R.F., Kaur B.
Rat brain tumor models in experimental neuro-oncology: the C6, 9L, T9, RG2, F98, BT4C, RT-2 and CNS-1 gliomas.
J. Neurooncol. 94:299-312(2009)
PubMed=25492533; DOI=10.3109/19401736.2014.982619
Gao J., Hu L., Peng H.-H., Shi J.-G.
The complete mitochondrial genome sequence of the rat C6 glioma cell line.
Mitochondrial DNA A. DNA Mapp. Seq. Anal. 27:2188-2189(2016)
DOI=10.5005/jp-journals-10082-02230
Kuduvalli S.S., Ramalakshmi O., Precilla S.D., Anitha T.S.
Evaluation of cell doubling time in C6 and Y79 cell lines based on seeding density.
SBV J. Basic Clin. Appl. Heatlh Sci. 2:146-149(2019)
PubMed=34917708; DOI=10.1016/j.dib.2021.107658; PMCID=PMC8666335
Silantyev A.S., Bukato O.N., Butenko I.O., Chernysheva A.A., Pobeguts O.V., Nosyrev A.E., Gurina O.I.
Proteomic dataset: profiling of glioma C6 and astrocytes rat cell lines before and after co-cultivation.
Data Brief 39:107658.1-107658.9(2021)
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文献和实验*发表【中文论文】请标注：由上海酶研生物科技有限公司提供；
*发表【英文论文】请标注：From Shanghai EK-Bioscience Biotechnology Co., Ltd.
【求助】求助：GFP或者萤光素酶稳定表达的U87或者C6细胞
adigao 由于我是药剂专业的，转染不是很熟悉，但是想用表达荧光的U87或者C6细胞做实验，哪位知道什么地方有卖的呢？或者能够定制呢？不胜感激！ 芭比妥酸 问问协和细胞中心，我只知道普通u87是700元 adigao 普通的细胞有的，但是都没有荧光标记的 aberrant adigao wrote: 普通的细胞
优于其他方法。 SABC-Cy3法激光扫描共聚焦显微镜下。C6细胞株波形蛋白免疫反应阳性细胞呈红色荧光(Hoochst 33255复染纲胞核，1200倍油镜放大)
固定后，PBS漂洗，1%锇酸固定1h，经包埋处理，超薄切片，在透射电镜（TEM）下观察细胞超微结构。 2、琼脂糖凝胶电泳成像：收集加入125I-UdR74KBq/ml+AUCG30μM后培养24h的C6细胞2×105个，2500rpm离心5min，去上清，加入裂解液，重悬细胞后，再加蛋白酶K消化30min，用等体积的平衡酚抽提两次，加入2倍体积的冰乙醇置-20℃2h，离心去上清，以75%的冰乙醇洗涤两次后，加双蒸水溶解DNA，取8μl DNA溶液及上样缓冲液2μl上样稀释，用1.5%的琼脂
技术资料