葡萄糖氧化酶来源于黑qu霉 *Sigma货号：G2133-1

一般描述

分子量：160 kDa（凝胶过滤） pI: 1865 消光系数：E1% = 16.7 (280 nm) 来自黑qu霉的葡萄糖氧化酶是由 2 个相等的亚基组成的二聚体，每个亚基的分子量为 80kDa
每个亚基含有一个黄素腺嘌呤二核苷酸部分和一个铁
该酶是含有 ~16％ 中性糖和 2％ 氨基糖的糖蛋白
该酶还含有 3 个半胱氨酸残基和 8 个 N-连接糖基化的潜在位点
葡萄糖氧化酶能够以不同的速率氧化 D-己醛糖、单脱氧-D-葡萄糖和甲基-D-葡萄糖
葡萄糖氧化酶的最佳 pH 为 5.5，虽然它的活性范围很宽，pH 4-7葡萄糖氧化酶对β-D-葡萄糖具有特异性，KM为33-110mM
Glucose oxidase does not require any activators, but it is inhibited by Ag+, Hg2+, Cu2+, phenylmercuric acetate, and p-chloromethylbenzoate. It is not inhibited by non-metallic SH reagents such as N-ethylmaleimide, iodoacetate, and iodoacetamide Glucose oxidase can be used for enzymatic determination of D-glucose in solutions. Due to the oxidation of β - D-glucose to D-gluconolactone and hydrogen peroxide by glucose oxidase, horseradish peroxidase is commonly used as a coupling enzyme for glucose determination. Although glucose oxidase has specificity for β - D-glucose, it can quantify D-glucose solutions because when enzymatic reactions consume β - D-glucose, α - D-glucose will become β - D-glucose