1-乙基-3-（3-二甲氨丙基）碳二亚胺盐酸盐 *Sigma

一般描述

1-乙基-3-（3-二甲基氨基丙基）碳酰二亚胺盐酸盐（EDC HCl）常被称为EDAC，它是化学和生物化学研究领域功能强大、应用广泛的水溶性试剂，在促进酰胺键的形成中起关键作用
在肽合成领域，EDC HCl高效催化羧基和胺基与氨基酸的偶联，从而促进形成具有特定序列和功能的肽骨架（peptide backbone）
这种特性在各种研究用肽的生产中有重要作用
除了肽以外，EDC HCl的影响还扩展到了免疫原的构建，它主动参与半抗原（诱发免疫反应的小分子）与载体蛋白的共价连接
这种参与有助于疫苗的研究和开发
The multifunctionality of EDC HCl is also reflected in its ability to modify nucleic acids, thereby labeling DNA and RNA through its 5 'phosphate group. This function facilitates imaging, tracking, and analysis of these key molecules, making significant contributions to advances in nucleic acid research. In addition, EDC HCl It can serve as an important biomolecule bridge, acting as a crosslinking agent to connect amine reactive NHS esters with carboxyl groups in biomolecules. This technology has significant value in protein coupling, as it can create hybrid molecules with novel characteristics and functions. The potential mechanism of EDC HCl involves the unstable intermediate formed by its reaction with carboxyl groups, which actively attracts amine partners. The subtle balance of this reaction emphasizes the need to optimize conditions to ensure efficient coupling. By stabilizing the intermediate and achieving a two-step coupling process, the assistance of N-hydroxysuccinimide (NHS) further enhances the ability of EDC HCl, leading to greater coupling. Flexibility and control, especially in handling complex biomolecules