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CD45 Mouse Monoclonal Antibody

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  • ¥1200 - 2150
  • EnkiLife
  • AMM03708
  • 2025年07月11日
  • WB;IHC
  • Mouse
  • Human;Mouse
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  • 企业认证

    • 详细信息
    • 文献和实验
    • 技术资料
    • 标记物

      Unconjugated

    • 适应物种

      Human;Mouse

    • 供应商

      武汉恩玑生命科技有限公司

    • 宿主

      Mouse

    • 应用范围

      WB;IHC

    • 规格

      50uL/100uL

    规格:50uL产品价格:¥1200.0
    规格:100uL产品价格:¥2150.0

    产品概述

    产品名称(Product Name)

    CD45 Mouse Monoclonal Antibody

    描述(Description)

    Primary antibody

    宿主(Host)

    Mouse

    应用(Application)

    WB,IHC-P

    种属反应性(Reactivity)

    Human,Mouse

     

    产品性能

    偶联物(Conjugation)

    Unconjugated

    修饰(Modification)

    Unmodified

    同种型(Isotype)

    IgG1

    克隆(Clonality)

    Monoclonal Antibody

    形式(Form)

    Liquid

    存放说明(Storage)

    Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.

    储存溶液(Buffer)

    Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide, pH 7.3.

    纯化方式(Purification)

    Affinity Purified

     

    免疫原

    基因名(Gene Name)

    PTPRC

    别名(Alternative Names)

    PTPRC; CD45; Receptor-type tyrosine-protein phosphatase C; Leukocyte common antigen; L-CA; T200; CD antigen CD45

    基因ID(Gene ID)

    5788

    蛋白ID(SwissProt ID)

    P08575

     

    产品应用

    稀释比(Dilution Ratio)

    WB: 1/500-1/1000 IHC: 1/50-1/100

    蛋白分子量(Molecular Weight)

    Calculated MW: 147 kDa; Observed MW: 180-240kDa

     

    研究背景

    The protein encoded by this gene is a member of the protein tyrosine phosphatase (PTP) family. PTPs are known to be signaling molecules that regulate a variety of cellular processes including cell growth, differentiation, mitosis, and oncogenic transformation. This PTP contains an extracellular domain, a single transmembrane segment and two tandem intracytoplasmic catalytic domains, and thus is classified as a receptor type PTP. This PTP has been shown to be an essential regulator of T- and B-cell antigen receptor signaling. It functions through either direct interaction with components of the antigen receptor complexes, or by activating various Src family kinases required for the antigen receptor signaling. This PTP also suppresses JAK kinases, and thus functions as a regulator of cytokine receptor signaling. Alternatively spliced transcripts variants of this gene, which encode distinct isoforms, have been reported.

     

    研究领域

    Immunology

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    图标文献和实验
    相关实验
    • Monoclonal Antibody Production

      and screening of the hybridomas: 1. Bleeding Mice (1) Place the mouse in a mouse restrainer. (2) Sterilize the tail with 70% ethanol. (3) With a razor blade,nip off the last 2 mm of the tip of the tail. (4) Using a milking motion,pull blood down and let

    • 单克隆抗体 monoclonal antibody

        指仅对一种抗原决定基的纯粹的抗体。这类抗体不可能通过将抗原 胞的 Hybridoma,在试管内制成了纯粹的单克隆抗体。用某种抗原使动物产生免疫感应,对应于抗原之不同部分可制成各种不同的抗体复合体。然而,因为一个产生抗体的细胞,只能产生一种抗体,所以骨髓肿瘤细胞与产生抗体细胞间的细胞杂种,可形成边产生抗体边增殖的 hybridoma,将每个杂种细胞进行克隆(无性繁殖系)培养,则一个克隆的细胞群只由单种类产生同一抗体的细胞所组成,这样得到的就是单克隆抗体。此抗体在测定

    • Monoclonal Antibody Production Protocol

      complete with MCM instead of PCM (= IMDM - m ), and keep it in the incubator for fused cells. Isolation of spleen cells Sacrifice mouse by cervical dislocation. Immerse mouse in 70% Ethanol. Remove spleen ( on the left side ) and transfer into a small

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