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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
Store at -20°C/-80°C upon receipt
- 保质期:
Generally, the shelf life of liquid form is 6 months at -20℃/-80℃. The shelf life of lyophilized form is 12 months at -20℃/-80℃.
- 英文名:
Neuropilin-2
- 库存:
50支
- 供应商:
艾普蒂
- 规格:
20μg/50μg
| 规格: | 20μg | 产品价格: | ¥1380.0 |
|---|---|---|---|
| 规格: | 50μg | 产品价格: | ¥2080.0 |
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文献和实验SQ Blood Mini Protocol for 50ul Clotted Blood
lysate and invert 10 time to mix throughly. Incubate the tube at 37°C for 5 minutes. 7. Place the tube on ice for 1 minute. Add 200 ul PCP buffer to the cell lysate. Vortex vigorously at high speed for 30 seconds to mix. Some protein clumps
SANDWICH ELISA FOR DETECTING SECRETE
with PBS. 3. Block remaining protein-binding sites by adding 200 ul/well of 1%BSA in PBS. Incubate 30 min at 37oC. 4. Wash 4X with PBS. 5. Add 50 ul of culture supernatant containing secreted ICAM-1. Include positive control wells of purified ICAM
for 64 mls for 128 mls stock soln 50 mM glucose 1.6 mls 3.2 mls 1M 25 mM Tris 8.0 0.8 mls 1.6 mls 1M pH 8.0 10 mM EDTA 0.64 mls 1.28 mls 0.5 M 4) Add 1.6 mls 10 mgs
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