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- 详细信息
- 技术资料
- 库存:
39
- 英文名:
Danshensu (Dan shen suan A)
- CAS号:
76822-21-4
- 供应商:
上海莼试
- 保存条件:
Store at 4°C
- 规格:
10mM*1mLinWater 50mg 100mg
产品描述:
分子式:

分子量:198.17
溶解度:H2O : 6.2 mg/mL (31.29 mM)
储存条件:Store at 4°C
General tips:For obtaining a higher solubility , please warm the tube at 37 ℃ and shake it in the ultrasonic bath for a while.
Shipping Condition:Evaluation sample solution : ship with blue ice All other available size: ship with RT , or blue ice upon request
Danshensu (DSS) significantly decreases the level of the marker enzymes (creatine kinase and lactate dehydrogenase) from the coronary effluents and myocardial infarction size. This could markedly contribute to the recovery of cardiac function after I/R injury. DSS also has ROS scavenging activity and boosts endogenous antioxidants such as SOD, CAT, MDA, GSH-PX and HO-1 activities by activating nuclear factor erythroid-2-related factor 2 (Nrf2) signaling pathway which is mediated by Akt and ERK1/2 in western blot analysis[2].Acute treatment with a single dose of danshensu in rats with normal tHcy does not change plasma tHcy. In contrast, danshensu significantly lowers tHcy in rats with elevated tHcy. The relatively higher cysteine and glutathione levels after treatment with danshensu indicates that its tHcy-lowering effect is via increased activity of the trans-sulphuration pathway[1].[1]. YG Cao, et al. Beneficial effects of danshensu, an active component of Salvia miltiorrhiza, on homocysteine metabolism via the trans-sulphuration pathway in rats. Br J Pharmacol. 2009 Jun; 157(3): 482-490. [2]. Yu J, et al. Danshensu protects isolated heart against ischemia reperfusion injury through activation of Akt/ERK1/2/Nrf2 signaling. Int J Clin Exp Med. 2015 Sep 15;8(9):14793-804.
商品属性:
注意事项:
抗逆滴加序列
每次向板内滴加抗原时,移液器滴头要与平面45度悬空,不要触碰到孔内的液体,由后向前依次滴加(即浓度由低往高滴加)。
抗感染反应期
抗原抗体在室温20~25℃下,必须反应30min以上,若环境温度低于室温,可将微量反应板置于恒温培养箱中,使二者充分反应。
旅游温度
磷酸盐缓冲液的pH值要在高压灭菌后进行滴定,往往在高压后pH值会有所改变,所以高压后再调一次pH值更为准确。磷酸盐缓冲液一经使用保存期不要超过3周。当pH<5.8时,红细胞会产生自凝现象;当pH>7.8时,图形洗脱加快,易造成肉眼观察产生误差;p H=7.2时,红细胞沉降最充分,图形最清晰。
当滴注 1%红细胞悬液时,应经常摇晃红细胞悬液,使红细胞均匀地分布在磷酸缓冲液中,以防止红细胞下降。
反应时间及温度
加入鸡红血球之后,反应板在室温(20~25℃)静置30~40min,对照孔血球下沉于孔底,即可判定结果。若室温达不到实验要求,需相应调整反应时间。当环境温度低于4℃时,红细胞发生自凝;高于37℃时,会发生反应物分离和红细胞溶血。
公司正在出售的产品:
使用方法:
1.本蛋白酶抑制剂混合物为100×的储存液,使用时按照1:100的比例加入到裂解液中(例如,1ml裂解液中加入10μl蛋白酶抑制剂混合物),混匀后即可使用。根据需要,0.5M的EDTA也按照1:100的比例加入到裂解液中(如用于检测金属蛋白酶活性,则不宜添加EDTA)。含有蛋白酶抑制剂混合物的裂解液宜现用现配,不宜配制后冻存待后续使用。
2. 待所需的抑制剂添加完毕混匀后,就可以开始进行哺乳动物组织的裂解和蛋白提取。
分子式:

分子量:198.17
溶解度:H2O : 6.2 mg/mL (31.29 mM)
储存条件:Store at 4°C
General tips:For obtaining a higher solubility , please warm the tube at 37 ℃ and shake it in the ultrasonic bath for a while.
Shipping Condition:Evaluation sample solution : ship with blue ice All other available size: ship with RT , or blue ice upon request
Danshensu (DSS) significantly decreases the level of the marker enzymes (creatine kinase and lactate dehydrogenase) from the coronary effluents and myocardial infarction size. This could markedly contribute to the recovery of cardiac function after I/R injury. DSS also has ROS scavenging activity and boosts endogenous antioxidants such as SOD, CAT, MDA, GSH-PX and HO-1 activities by activating nuclear factor erythroid-2-related factor 2 (Nrf2) signaling pathway which is mediated by Akt and ERK1/2 in western blot analysis[2].Acute treatment with a single dose of danshensu in rats with normal tHcy does not change plasma tHcy. In contrast, danshensu significantly lowers tHcy in rats with elevated tHcy. The relatively higher cysteine and glutathione levels after treatment with danshensu indicates that its tHcy-lowering effect is via increased activity of the trans-sulphuration pathway[1].[1]. YG Cao, et al. Beneficial effects of danshensu, an active component of Salvia miltiorrhiza, on homocysteine metabolism via the trans-sulphuration pathway in rats. Br J Pharmacol. 2009 Jun; 157(3): 482-490. [2]. Yu J, et al. Danshensu protects isolated heart against ischemia reperfusion injury through activation of Akt/ERK1/2/Nrf2 signaling. Int J Clin Exp Med. 2015 Sep 15;8(9):14793-804.
商品属性:
| 货号 | CS-01Y65483 | 规格 | 10mM*1mLinWater 50mg 100mg |
| CAS号 | 76822-21-4 | 分子量 | 198.17 |
| 含量 | >98.00% | 别名 | N/A |
| 分子式 | 化学名 | N/A | |
| 产地 | 国产 | 用途 | 仅供科研研究实验 |
抗逆滴加序列
每次向板内滴加抗原时,移液器滴头要与平面45度悬空,不要触碰到孔内的液体,由后向前依次滴加(即浓度由低往高滴加)。
抗感染反应期
抗原抗体在室温20~25℃下,必须反应30min以上,若环境温度低于室温,可将微量反应板置于恒温培养箱中,使二者充分反应。
旅游温度
磷酸盐缓冲液的pH值要在高压灭菌后进行滴定,往往在高压后pH值会有所改变,所以高压后再调一次pH值更为准确。磷酸盐缓冲液一经使用保存期不要超过3周。当pH<5.8时,红细胞会产生自凝现象;当pH>7.8时,图形洗脱加快,易造成肉眼观察产生误差;p H=7.2时,红细胞沉降最充分,图形最清晰。
当滴注 1%红细胞悬液时,应经常摇晃红细胞悬液,使红细胞均匀地分布在磷酸缓冲液中,以防止红细胞下降。
反应时间及温度
加入鸡红血球之后,反应板在室温(20~25℃)静置30~40min,对照孔血球下沉于孔底,即可判定结果。若室温达不到实验要求,需相应调整反应时间。当环境温度低于4℃时,红细胞发生自凝;高于37℃时,会发生反应物分离和红细胞溶血。
公司正在出售的产品:
| Recombnant Human CD304/NRP1, N-H | 溶血磷脂酸受体蛋白3/EDG7封闭多肽 |
| 磷酸化微管相关蛋白封闭多肽 | phospho-FYN (Tyr530) Antibody Blocking Peptide |
| NDUFC2蛋白封闭多肽 | Histone H2B Antibody Blocking Peptide |
| 土霉素鸡卵白蛋白偶联物 | Tropomodulin 4 Antibody Blocking Peptide |
| 钠依赖性脯氨酸转运PROT封闭多肽 | ILDR1 Antibody Blocking Peptide |
| 内脂素/内脏脂肪素/前B细胞克隆增强因子1封闭多肽 | SIX5 Antibody Blocking Peptide |
| 溶质载体家族12成员8封闭多肽 | FAM83F Antibody Blocking Peptide |
| 磷酸化原癌基因c-Raf封闭多肽 | 单加氧酶X封闭多肽 |
| 磷酸化酪氨酸蛋白激酶Fyn/同步原癌基因封闭多肽 | phospho-c-Raf (Thr269) Antibody Blocking Peptide |
| 组蛋白H2B封闭多肽 | LC12A8 Antibody Blocking Peptide |
| 免疫球蛋白样结构域受体1封闭多肽 | NAMPT/Visfatin-1 Antibody Blocking Peptide |
| 原肌球蛋白调节蛋白4封闭多肽 | SLC6A7 Antibody Blocking Peptide |
| 糖尿病相关同源结构域蛋白SIX5封闭多肽 | Danshensu (Dan shen suan A)Oxytetracycline/OVA |
| FAM83F蛋白封闭多肽 | NDUFC2 Antibody Blocking Peptide |
| LPAR3 Antibody Blocking Peptide | phospho-Tau protein (Thr548) Antibody Blocking Peptide |
1.本蛋白酶抑制剂混合物为100×的储存液,使用时按照1:100的比例加入到裂解液中(例如,1ml裂解液中加入10μl蛋白酶抑制剂混合物),混匀后即可使用。根据需要,0.5M的EDTA也按照1:100的比例加入到裂解液中(如用于检测金属蛋白酶活性,则不宜添加EDTA)。含有蛋白酶抑制剂混合物的裂解液宜现用现配,不宜配制后冻存待后续使用。
2. 待所需的抑制剂添加完毕混匀后,就可以开始进行哺乳动物组织的裂解和蛋白提取。
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Danshensu (Dan shen suan A)
¥600 - 3200








