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- 2026年03月13日
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- 详细信息
- 询价记录
- 文献和实验
- 技术资料
- 英文名：
Rat brain astrocyte cell line
- 库存：
10
- 供应商：
欣润生物
- 肿瘤类型：
NO
- 细胞类型：
永生化
- ATCC Number：
11222
- 品系：
SD大鼠
- 组织来源：
大脑皮层
- 相关疾病：
无
- 物种来源：
大鼠
- 免疫类型：
不详
- 细胞形态：
成纤维细胞样
- 是否是肿瘤细胞：
否
- 器官来源：
大脑
- 运输方式：
常温
- 年限：
新生鼠
- 生长状态：
贴壁生长
- 规格：
T25方瓶
永生化大鼠脑星形胶质细胞简介：
产品名称：大鼠脑星形胶质细胞系、永生化大鼠鼠脑星形胶质细胞、大鼠脑星形细胞系、永生化大鼠脑星形细胞
产品描述：永生化大鼠脑星形胶质细胞、永生化大鼠脑星形胶质细胞是哺乳动物脑内分布最广泛的一类细胞，也是胶质细胞中体积较大的一种。用经典的金属浸镀技术（银染色）显示此类胶质细胞呈星形，从胞体发出许多长而分支的突起，伸展充填在神经细胞的胞体及其突起之间，起支持和分隔神经细胞的作用。小鼠脑星形胶质细胞系、永生化小鼠脑星形胶质细胞还具有营养作用，协助神经元的代谢，星形胶质细胞通过血管周足和突起连接毛细血管与神经元，对神经元起到运输营养物质和排除代谢产物的作用。
产品货号：IR3007
产品类型: 永生化细胞系
传代能力: 可以传代30代左右
产品形态: 成纤维细胞样
培养基：大鼠脑星形胶质细胞系、永生化大鼠脑星形胶质细胞专用完全培养基
支原体质控：检测呈阴性
产品培养条件：37℃，5%CO2
发货方式：T25瓶子常温发货
货期：7天左右货期
GFAP抗体免疫荧光染色鉴定
Ethanol effects on phospholipids and triacylglycerols of rat brain astrocyte cell line
A homotypic population of rat brain astrocyte (RBA-1) cell line was used to investigate the effects of ethanol on uptake of [3H]-arachidonic acid and incorporation of label into phospholipids and neutral lipids in the cell. Incubation of the cells with [3H]-arachidonic acid resulted in labeling of most phospholipids and triacylglycerols, although individual phospholipids exhibited different rates for acquiring the label. Labeled arachidonate was first incorporated into phosphatidylcholines and phosphatidylinositols prior to equilibriation with other phospholipids. In short term incubation (8 hr), ethanol (128-255 mM) enhanced the incorporation of labeled arachidonate into most phospholipids. In addition, ethanol at 128 mM enhanced the labeling of the triacylglycerols, and the difference was greater with increasing time of ethanol exposure. It is concluded that ethanol enhanced the uptake and incorporation of polyunsaturated fatty acids into the cell membrane lipids. This study further indicates the feasibility of using the RBA-1 cells in culture to probe the effects of ethanol
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- 作者
- 内容
- 询问日期
文献和实验Chicken intestinal epithelial cells were obtained from NEWGAINBIO company. Cells were cultured on 37℃, with 5% CO2, in the Ham’s F-12 Nutrient (DMEM/12) that contained the following supplementations: fetal bovine serum (5%), in-sulin (5 µg/mL), transferrin (5 µg/mL), selenium (5 ng/mL), epidermal growth factor (5 ng/mL) and penicillin-streptomycin (100–100 U/mL) for cell culturing (full DMEM/12). Experiments were performed with chicken intestinal epithelial cells and working solutions were prepared with plain DMEM/12 without supplementation. For the investigations, cells were seeded onto 96-well, 24-well or 6-well polystyrene cell culture plates.
Primary hVICs (passage 2) were cultured to 50–60% confluence and infected with pGMLV-SV40T-puro lentivirus (NewgainBio, Wuxi, China) at a multiplicity of infection of 80 supplemented with 5 µg/mL polybrene (Sigma-Aldrich, Buchs, Switzerland).
Tissue was cultured until cells became visible around the tissue, and when the fusion reached 90% (FIGURE 1A) §ask ¦lled with the prepared culturing medium was sent to the company for further immortalisation. Cell immortalisation was done for cell stability and longer-term use. Immortalised cells were cultured with 10% FBS and 1% PS in the DMEM medium. After the cells multiplied and merged, they were routinely passed and grown ( NEWGAINBIO Inc. Wuxi, Jiangsu, China) (FIGURE 1B-C).
Mouse primary cultured renal vascular ECs and VSMCs were obtained from Newgainbio company, which were tested by Factor VIII and α-smooth muscle actin (α-SMA), the marker of ECs and VSMCs. RNeasy Mini Kit was used for RNA extraction, and the above protocols were repeated.
Porcine primary colon epithelial cells (Newgainbio company, Wuxi,China) were cultured in Dulbecco's Modified Eagle's Medium (Solarbio, Beijing, China) containing 10 % fetal bovine serum (BioInd, Kiryat shmona, Lsrael) at 37 ◦C and 5 % CO2 humidity.
平行排列，经S-100免疫组织化学染色呈阳性反应。增殖分裂的雪旺细胞可用于进一步传代培养，也可进行冷冻保存备用。 (二)星形胶质细胞 星形胶质细胞是胶质细胞中体积最大，数量最多的一种，胞体呈星形，核大，呈卵圆形，染色质稀少，星形胶质细胞分两类，一类为原浆性星形胶质细胞(protoplasmic astrocyte)，其突起短粗，分枝多。另一种为纤维性星形胶质细胞(fibrous astrocyte)，它的突起细长，分枝少。纤维性星形胶质细胞是与少突胶质细胞源自同一前体细胞。星形胶质细胞
递呈。 ④星形细胞增殖加速与脑受损后的瘢痕形成及MS的硬化斑均有密切的关系。 以上事实说明，星形细胞可视为脑内的免疫辅助细胞，介导中枢神经系统内部的神经、免疫内分泌相互联系。 （2）小胶质细胞：现已证明，脑内的小胶质细胞是由骨髓单核细胞系来源并迁入和定居于中枢神经系统的。与外周组织中的Mφ类似，小胶质细胞表面的CR3受体和Fc受体，并表达低水平的CD4抗原、MHc Ⅱ类抗原、转铁蛋白受体和B细胞共同抗原。上胶质细胞具有多方面免疫相关功能，参与神经系统的发育和重塑，调节神经
图 （采自Escourolle） （二）神经胶质细胞 神经胶质细胞包括星形胶质细胞、少突胶质细胞和室管膜细胞，其总数是神经元的5倍，其基本病变概括如下： 1．星形胶质细胞 （1）星形胶质细胞增生：常见的致病原因如缺氧、低血糖、感染、中毒等均能引起脑组织的损伤而导致星形胶质细胞增生。反应性星形胶质细胞增生是脑组织损伤的修补愈合反应，主要表现为纤维型星形胶质细胞增生，最后成为胶质瘢痕，胶质纤维酸性蛋白（GFAP）染色呈强阳性（图16-4）。胶质瘢痕与纤维瘢痕不同之处在于星形胶质细胞
技术资料
