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Wnt3/Wnt3蛋白Recombinant Mouse P

roto-oncogene Wnt-3 (Wnt3)重组蛋白Proto-oncogene Int-4蛋白
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  • ¥1836 - 14796
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  • CSB-EP026135MOb0
  • 2025年07月11日
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    • 详细信息
    • 文献和实验
    • 技术资料
    • 保存条件

      Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

    • 保质期

      Generally, the shelf life of liquid form is 6 months at -20℃/-80℃. The shelf life of lyophilized form is 12 months at -20℃/-80℃.

    • 英文名

      Recombinant Mouse Proto-oncogene Wnt-3 (Wnt3)

    • 库存

      200

    • 供应商

      武汉华美生物工程有限公司

    • 规格

      1mg/100μg/20μg

    规格:1mg产品价格:¥14796.0
    规格:100μg产品价格:¥3468.0
    规格:20μg产品价格:¥1836.0

    纯度:

    Greater than 85% as determined by SDS-PAGE.

    基因名:

    Wnt3

    Uniprot No.:

    P17553

    别名:

    Proto-oncogene Int-4

    种属:

    Mus musculus (Mouse)

    蛋白长度:

    Full Length of Mature Protein

    来源:

    E.coli

    分子量:

    43 kDa

    表达区域:

    22-355aa

    氨基酸序列:

    GYPIWWSLALGQQYTSLASQPLLCGSIPGLVPKQLRFCRNYIEIMPSVAEGVKLGIQECQHQFRGRRWNCTTIDDSLAIFGPVLDKATRESAFVHAIASAGVAFAVTRSCAEGTSTICGCDSHHKGPPGEGWKWGGCSEDADFGVLVSREFADARENRPDARSAMNKHNNEAGRTTILDHMHLKCKCHGLSGSCEVKTCWWAQPDFRAIGDFLKDKYDSASEMVVEKHRESRGWVETLRAKYALFKPPTERDLVYYENSPNFCEPNPETGSFGTRDRTCNVTSHGIDGCDLLCCGRGHNTRTEKRKEKCHCVFHWCCYVSCQECIRIYDVHTCK

    蛋白标签:

    N-terminal 10xHis-tagged

    产品提供形式:

    Liquid or Lyophilized powder

    缓冲液:

    If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.

    货期:

    13-23 business days

    注意事项:

    Repeated freezing and thawing is not recommended. Store working aliquots at 4℃ for up to one week.

    功能:

    Ligand for mbers of the frizzled family of seven transmbrane receptors. Wnt-3 and Wnt-3a play distinct roles in cell-cell signaling during morphogenesis of the developing neural tube.

    内毒素:

    Not test.

    SDS-PAGE:

    (Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.

    LC-MS Image Description/Western Blot:

    /

    Product types:

    Developed Protein

    Biological_Activity:

    /

    Research Areas:

    Others

    Reconstitution:

    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.

    Reference:

    Wnt-3, a gene activated by proviral insertion in mouse mammary tumors, is homologous to int-1/Wnt-1 and is normally expressed in mouse embryos and adult brain.Roelink H., Wagenaar E., Lopes da Silva S., Nusse R.Proc. Natl. Acad. Sci. U.S.A. 87:4519-4523(1990)

    Function:

    Ligand for members of the frizzled family of seven transmembrane receptors. Wnt-3 and Wnt-3a play distinct roles in cell-cell signaling during morphogenesis of the developing neural tube.

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    图标文献和实验
    该产品被引用文献
    Wnt-3, a gene activated by proviral insertion in mouse mammary tumors, is homologous to int-1/Wnt-1 and is normally expressed in mouse embryos and adult brain.Roelink H., Wagenaar E., Lopes da Silva S., Nusse R.Proc. Natl. Acad. Sci. U.S.A. 87:4519-4523(1990)
    相关实验
    • v468 Chapter 2 Wnt活性蛋白分离及应用

      dishes (e.g., Corning® CellSTACK®). 6. CO 2 incubator and biosafety cabinet. 7. Filter bottle, 0.5–1 L, 0.2-μm pore size (Corning or equivalent). 2.2. Preparation of Wnt3A CM for Fractionation 1. 20% (v/v) Triton X-100. 2. 1 M Tris-HCl, pH

    • 【求助】激活wnt的几种方法

      miaoduan 本人刚刚开始做实验,有关wnt通路的检测,想请教激活wnt通路的方法,看资料主要有三种: 1,加wnt蛋白 2,LiCl抑制gsk3β 3,转染wnt质粒或者点突变的β-catenin质粒 不知哪种方法可行,具体如何操作呢 非常感谢!!! edwardellen 加wnt3a,看过一篇CANCER CELL是这样激活的 liubodoctor

    • 三句话读懂一篇 CNS:百岁老人的长寿秘诀;雄性激素越高越有

      物 ATPase7/CDC50C 在疟原虫按蚊感染和传播过程中的功能和分子机制,揭示了疟原虫躲避按蚊宿主免疫识别和清除的潜在新机制。疟原虫每年导致全球数十万人死伤,望其早日被消灭!图 3:来源 Science Advances4. Nature Communications: 解析蛋白 Wntless 与 Wnt3a 复合物结构Wnt 蛋白是一类蛋白质信号分子,Wnt 信号转导活动异常导致多种疾病。2021 年 7 月 27 日,西湖大学马丹与周强课题组在 Nature Communications 上发表

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    文献支持
    Wnt3/Wnt3蛋白Recombinant Mouse Proto-oncogene Wnt-3 (Wnt3)重组蛋白Proto-oncogene Int-4蛋白
    ¥1836 - 14796