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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
收到后储存在 -20°C/-80°C,必须分装多次使用。避免重复冻融循环。
- 保质期:
液体形式的保质期在-20°C/-80°C下为6个月,冻干形式的保质期在-20°C/-80°C下为12个月。
- 英文名:
Wnt3
- 库存:
88
- 供应商:
澳培赛生物科技(上海)有限公司
- 规格:
1mg/100ug/20ug
| 规格: | 1mg | 产品价格: | ¥13316.0 |
|---|---|---|---|
| 规格: | 100ug | 产品价格: | ¥3121.0 |
| 规格: | 20ug | 产品价格: | ¥1652.0 |
[ 储存条件 ]
收到后储存在 -20°C/-80°C,必须分装多次使用。避免重复冻融循环。
[ 有效期 ]
液体形式的保质期在-20°C/-80°C下为6个月,冻干形式的保质期在-20°C/-80°C下为12个月。
[ 重要说明 ]
This product is for scientific research purposes only and should not be used for clinical diagnosis or any other procedures, otherwise the consequences will be borne by oneself. The instruction manual shall be based on the actual delivery manual!
该产品仅供科研使用,不得用于临床诊断或任何其他程序,否则后果自负。说明书以实际到货说明书为准!
[ Identification ]
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文献和实验1. Mouse L-cells (ATCC CCL-1.3 or CRL-2648). 2. Cell culture medium: Same as Section 2.1.2. 3. 96-well tissue culture plate (flat well). 4. Wnt3A samples (CM, fractions from Sections. 2.3 and 2.4 ). 5. PBS. 6. Lysis Buffer: 50 mM Tris-HCl, pH
v468.chapter 1 经典 Wnt/b-Catenin 信号通路 (英文完整版)
-1 a proto-oncogene) and revealed Wnt-1to be a secreted cysteine-rich protein with the potential to act asa signalling molecule. However, the real breakthrough in linking thisgene to a signalling pathway was made by fly geneticists, who demonstrated
v468. chapter 4 WNT 细胞通路 检测细胞GSK3活性与表达水平
phosphate-buffered saline (PBS) containing 0.1% (w/v)bovine serum albumin (BSA) at a concentration of 50 μg/mLand stored in aliquots at –20°C (see Note 2 ). 3. Mouse L cells that express and secrete Wnt3a (ATCC, Manassas,VA; #CRL-2647) and the control L
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