TM40L rabbit pAb

Immunochemical Detection of a Fluorophore Derived from the Lipid Peroxidation Product 4-Hydroxy-2-Nonenal and Lysine

= NAL), appears to form upon oxidative cyclization of the nonfluorescent 2:1 lysine-HNE Michael adduct-Schiff base cross-link (Scheme 1). Polyclonal antibody (PAb) to the NAL-HNE fluorophore was raised in rabbit and found to be highly specific

Mapping Protein Distributions on Polytene Chromosomes by Immunostaining

To immunostain using enzyme-coupled secondary antibodies: i. Rinse slides in PBS and remove excess solution. ii. Add 40 µL of secondary antibody (e.g., anti-rabbit IgG [Fc] HRP conjugate, 1:100 dilution

ChIP 实验面面观，博士师兄吐血总结推荐

了 ChIP 数据的质量和可信度。而 CST 生产的 ChIP 抗体多数是兔单抗，相比传统多克隆抗体（pAb） 和鼠单抗，特异性会大大提高，同时也保持了最高的亲和力，保证了 ChIP 数据的高质量和 ChIP DNA 富集的高效性。相关内部检测数据，请参看 http://www.cst-c.com.cn/resources.html 和相关文章《你看到的组蛋白修饰是不是真的？》。然后加入 30μl 的 ChIP 级蛋白 G 微珠（强烈推荐使用 CST 的#9006 磁性微珠，便于操作），在 4°C 的转子