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- 详细信息
- 文献和实验
- 技术资料
- 英文名:
Gallium (Ga) Standard Solution: 镓标准溶液
- 库存:
1
- 供应商:
甄准生物
- 规格:
5 x 10 mL
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文献和实验of water to prepare a 1M piperidine solution. Place the tube containing the formic acid reaction on ice, add all 150 μl of the diluted (1M) piperidine solution and incubate for 30 min at 90C. Place this reaction on ice for 5min, add 1ml
Standard hybridization technique
The standard solution typically used for both pre-hybridisation and hybridisation is based
as otherwise all available Mg2+ is chelated out of solution and enzyme reactivity is adversely affected. Any increase in dNTPs over 200uM means [Mg2+] should be re-optimised.AVOID USING EDTA-CONTAINING BUFFERS AS EDTA CHELATES Mg2+Low primer, target, Taq, and nucleotide
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