Mouse IgM Isotype Control Anti

Isotype-specific immunostaining with mouse primary antibodies on mouse tissue

) Briefly, substitute your anti H+L pan-reactive secondary antibody with an anti-isotype heavy chain specific secondary. Be aware of possible odd crossreactivties (e.g. many goat anti mouse IgG 1 heavy chain do stain rat IgG 2a

Isotype Determination of Monoclonal Antibodies by Immunodiffusion

antibodies are allowed to diffuse toward anti-mouse isotype specific antisera in an agarose gel. In a positive reaction, a precipitin line will develop in the agarose between two wells. A monoclonal antibody should show only one heavy chain and one light

Rapid Isotyping of Mouse Monoclonal Antibodies

, isotyping is useful to control lot to lot consistency, detect contamination by another antibody, and identify hybridoma switch variants (see Notes 2 –8 for further comment on applications). Table 1  Isotype-Dependent Properties of Monoclonal