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- 上海莼试
- CS-01Y71663
- 国产
- 2025年07月11日
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- 详细信息
- 询价记录
- 文献和实验
- 技术资料
- 库存：
21
- 英文名：
Acivicin hydrochloride
- CAS号：
161922-40-3
- 供应商：
上海莼试
- 保存条件：
4°C, stored under argon
- 规格：
5mg 10mg 25mg 50mg 100mg
| 货号 | CS-01Y71663 | 规格 | 5mg 10mg 25mg 50mg 100mg |
| CAS号 | 161922-40-3 | 分子量 | 215.03 |
| 含量 | >99.00% | 别名 | N/A |
| 分子式 | C5H8Cl2N2O3 | 化学名 | N/A |
| 产地 | 国产 | 用途 | 仅供科研研究实验 |
分子式:C5H8Cl2N2O3
分子量:215.03
溶解度:N/A
储存条件:4°C, stored under argon
General tips:For obtaining a higher solubility , please warm the tube at 37 ℃ and shake it in the ultrasonic bath for a while.
Shipping Condition:Evaluation sample solution : ship with blue ice All other available size: ship with RT , or blue ice upon request
Acivicin hydrochloride (AT-125 hydrochloride), a natural product produced by Streptomyces sviceus, is a γ-glutamyl transpeptidase (GGT) inhibitor. Acivicin hydrochloride can across the blood-brain barrier and has anti-cancer, anti-parasitic properties[1][2].Acivicin hydrochloride (AT-125 hydrochloride; 0.1-50 μM; 5 days) has an IC50 of 0.7 μM in human HepG2 cells[1].Acivicin hydrochloride (AT-125 hydrochloride; 0.1-50 μM; 5 days; for 4 days) reduces urinary gamma-GT by 70-78%[1]. Animal Model: Male pigmented Long-Evans rats weighed between 250 g and 300 g exposed to Tolune[1][1]. Kreuzer J, et al. Target discovery of acivicin in cancer cells elucidates its mechanism of growth inhibition?Electronic supplementary information (ESI) available: Synthesis, cloning, protein expression, purification and biochemical assays. Chem Sci. 2014 Dec 1;6(1):237-245. Epub 2014 Sep 26. [2]. Chikhale EG, et al. Carrier-mediated transport of the antitumor agent acivicin across the blood-brain barrier. Biochem Pharmacol. 1995 Mar 30;49(7):941-5. [3]. Delphine Waniusiow, et al. Toluee-induced hearing loss in acivicin-treated rats. Neurotoxicol Teratol. May-Jun 2008;30(3):154-60.
注意事项：
抗逆滴加序列
每次向板内滴加抗原时，移液器滴头要与平面45度悬空，不要触碰到孔内的液体，由后向前依次滴加(即浓度由低往高滴加)。
抗感染反应期
抗原抗体在室温20~25℃下，必须反应30min以上，若环境温度低于室温，可将微量反应板置于恒温培养箱中，使二者充分反应。
旅游温度
磷酸盐缓冲液的pH值要在高压灭菌后进行滴定，往往在高压后pH值会有所改变，所以高压后再调一次pH值更为准确。磷酸盐缓冲液一经使用保存期不要超过3周。当pH<5.8时，红细胞会产生自凝现象;当pH>7.8时，图形洗脱加快，易造成肉眼观察产生误差;p H=7.2时，红细胞沉降最充分，图形最清晰。
当滴注 1%红细胞悬液时，应经常摇晃红细胞悬液，使红细胞均匀地分布在磷酸缓冲液中，以防止红细胞下降。
反应时间及温度
加入鸡红血球之后，反应板在室温(20~25℃)静置30~40min，对照孔血球下沉于孔底，即可判定结果。若室温达不到实验要求，需相应调整反应时间。当环境温度低于4℃时，红细胞发生自凝;高于37℃时，会发生反应物分离和红细胞溶血。
使用方法：
1.本蛋白酶抑制剂混合物为100×的储存液，使用时按照1:100的比例加入到裂解液中(例如，1ml裂解液中加入10μl蛋白酶抑制剂混合物)，混匀后即可使用。根据需要，0.5M的EDTA也按照1:100的比例加入到裂解液中（如用于检测金属蛋白酶活性，则不宜添加EDTA）。含有蛋白酶抑制剂混合物的裂解液宜现用现配，不宜配制后冻存待后续使用。
2. 待所需的抑制剂添加完毕混匀后，就可以开始进行哺乳动物组织的裂解和蛋白提取。
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文献和实验Guanidine Hydrochloride Purification of Proteins From SDS
Pepstatin A (2.0 mg/ml)10 ml Leupeptin (1.0 mg/ml)10 ml Aprotinin (5.0 mg/ml)(Protease Inhibitor Cocktail Sigma #P8340)Denaturation Buffer6 M guanidine hydrochloride 57.3 g G-HCl20 mM Hepes 7.9 2 ml 1M Hepes pH 7.910 mM KCl 0.074 g KCl0.5 mM DTT 100 ml 0.5 M
Guanidine Hydrochloride Purification of Proteins From SDS PAGE
(Sigma #P7626) 10 ml Pepstatin A (2.0 mg/ml) 10 ml Leupeptin (1.0 mg/ml) 10 ml Aprotinin (5.0 mg/ml) (Protease Inhibitor Cocktail Sigma #P8340) Denaturation Buffer 6 M guanidine hydrochloride 57.3 g G-HCl 20 mM Hepes 7.9 2 ml 1M Hepes pH 7.9 10 mM
Packed column subcritical and/or supercritical fluid chromatography (p-sub or pSFC) has been used as a powerful chiral separation technique, whereby a mobile phase produces low viscosity, a high diffusion coefficient, and a solvating power. P
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