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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
-20℃ to -80℃
- 保质期:
12个月
- 英文名:
PP1B, Active
- 库存:
99
- 供应商:
北京义翘神州科技股份有限公司
- 规格:
10 µg
蛋白名称:PP1B, Active
蛋白构建:Recombinant full length human PP1B was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag.
表达宿主:Baculovirus-Insect Cells
蛋白分子量:~60 kDa
蛋白NP号:NM_002709
蛋白氨基酸序列:Full Length
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文献和实验Quantification of Active Caspase-3 and Active Caspase-8 in Microglia Cells
During microglia activation the levels of active caspase-3, caspase-7 and caspase-8 are increased, which leads to the transcription of proinflammatory cytokines and factors. As such, the induction of caspase activity in microglia can be used
Immunoblotting for Active Caspase-1
Immunoblotting for caspase-1 is the gold-standard method of detecting inflammasome activation. In contrast to IL-1β-based readouts, it can be used in an experimental setup independent of de novo gene expression. Here, we present protocols
that need to be optimized, including the choice of antibody, the conditions for shearing chromatin using sonication and the analysis of immunoprecipitated DNA. The ChIP-IT Kit from Active Motif provides a complete solution to all potential problems associated with the ChIP
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