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- 详细信息
- 文献和实验
- 技术资料
- 免疫原:
Synthesized peptide derived from internal of Human BAX.
- 亚型:
/
- 形态:
Liquid
- 保存条件:
Upon receipt, store at -20℃ or -80℃. Avoid repeated freeze.
- 克隆性:
Polyclonal
- 标记物:
/
- 适应物种:
Human,Mouse,Rat
- 保质期:
6个月
- 抗原来源:
Homo sapiens (Human)
- 目录编号:
Q07814
- 级别:
优
- 库存:
200
- 供应商:
武汉华美生物工程有限公司
- 宿主:
Rabbit
- 应用范围:
ELISA,WB,IHC,IF;WB:1:500-1:3000,IHC:1:50-1:100,IF:1:100-1:500
- 浓度:
>95%,The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
- 靶点:
/
- 抗体英文名:
BAX Antibody
- 抗体名:
/
- 规格:
100μl
保存缓冲液
/功能
The protein encoded by this gene belongs to the BCL2 protein family. BCL2 family members form hetero- or homodimers and act as anti- or pro-apoptotic regulators that are involved in a wide variety of cellular activities. This protein forms a heterodimer with BCL2, and functions as an apoptotic activator. This protein is reported to interact with, and increase the opening of, the mitochondrial voltage-dependent anion channel (VDAC), which leads to the loss in membrane potential and the release of cytochrome c. The expression of this gene is regulated by the tumor suppressor P53 and has been shown to be involved in P53-mediated apoptosis. Multiple alternatively spliced transcript variants, which encode different isoforms, have been reported for this gene.Oltvai Z.N., Cell 74:609-619(1993).
Apte S.S., Genomics 26:592-594(1995).
Shi B., Biochem. Biophys. Res. Commun. 254:779-785(1999).
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文献和实验脾细胞与小鼠骨髓瘤细胞(P3-X63/Ag8)融合,融合的细胞既获得了亲代脾细胞分泌特异性抗体的特性,又具有骨髓瘤细胞大量繁殖的能力,成为一种既能分泌特异性抗体又能长命的杂交瘤细胞。该技术为抗体的分子生物学研究提供了全新的手段。极大地促进了免疫学,遗传学,分子生物学的快速发展。 但传统抗体目前也面临诸多问题,如: 每使用一管新抗体都需要进行滴定测试; 多种同型对照,实验设计繁琐; 需要添加Fc阻断试剂; 抗体不佳,阳性细胞群不明显; 抗体作为细胞生物学和生物化学中最重要的试剂之一,科学界每年在这类
luozhifeng339 请教各位达达,我准备做EMSA,如果做SUPER-SHIFT的话,该选择原装抗体还是分装的抗体呢,盼不吝赐教! wanglinling 看到一篇EMSA的文章,不知道里面有没有回答你的问题哈,不过英文的值得阅读哈。 GEL SUPER-SHIFT ASSAY/ EMSA Materials/Reagents: See Gel-shift assay Procedure
在免疫反应方面,基于抗原的刺激在体内形成、并与抗原作特异性结合的蛋白质之总称。其化学实质是免疫球蛋白。抗体,按照抗原抗体反应的类型可分别称为沉淀素、凝集素、溶血素、溶菌素、抗毒素、中和抗体等,但这些并不等于有相应的不同抗体存在,一般多只表明抗体反应的多样性而已。抗体在血清中含量最多(体液抗体=humoral antibody);含有某特定抗体的血清称为抗血或免疫血清。
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