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人ATPSCKMT重组蛋白Protein N-lysine

methyltransferase FAM173B;hFAM173BHuman ATP synthase subunit C lysine N-methyltransferase(ATPSCKMT)蛋白
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  • ¥9720
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  • CN
  • CSB-CF761288HU
  • 2026年01月20日
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    • 详细信息
    • 文献和实验
    • 技术资料
    • 保存条件

      We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃.

    • 保质期

      Generally, the shelf life of liquid form is 6 months at -20℃/-80℃. The shelf life of lyophilized form is 12 months at -20℃/-80℃.

    • 英文名

      Recombinant Human ATP synthase subunit C lysine N-methyltransferase (ATPSCKMT)

    • 库存

      200

    • 供应商

      武汉华美生物工程有限公司

    • 规格

      20μg


    英文名称:

    Recombinant Human ATP synthase subunit C lysine N-methyltransferase (ATPSCKMT)

    品名简称:

    Recombinant Human ATPSCKMT protein

    货号:

    CSB-CF761288HU

    规格:

    20μg

    价格:

    9720

    LC-MS/Western Blot:

    /

    SDS-PAGE:

    (Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.

    纯度:

    Greater than 85% as determined by SDS-PAGE.

    内毒素:

    Not test

    生物活性:

    /

    基因名:

    ATPSCKMT

    Uniprot No.:

    Q6P4H8

    别名:

    Protein N-lysine methyltransferase FAM173B;hFAM173B

    种属:

    Homo sapiens (Human)

    蛋白长度:

    Full Length

    来源:

    in vitro E.coli expression system

    分子量:

    32.2 kDa

    表达区域:

    1-233aa

    氨基酸序列:

    MEGGGGIPLETLKEESQSRHVLPASFEVNSLQKSNWGFLLTGLVGGTLVAVYAVATPFVTPALRKVCLPFVPATTKQIENVVKMLRCRRGSLVDIGSGDGRIVIAAAKKGFTAVGYELNPWLVWYSRYRAWREGVHGSAKFYISDLWKVTFSQYSNVVIFGVPQMMLQLEKKLERELEDDARVIACRFPFPHWTPDHVTGEGIDTVWAYDASTFRGREKRPCTSMHFQLPIQA

    蛋白标签:

    N-terminal 10xHis-tagged

    产品提供形式:

    Liquid or Lyophilized powder

    缓冲液:

    If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.

    储存条件:

    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.

    保质期:

    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20℃/-80℃. The shelf life of lyophilized form is 12 months at -20℃/-80℃.

    货期:

    18-23 business days

    注意事项:

    Repeated freezing and thawing is not recommended. Store working aliquots at 4℃ for up to one week.

    功能1:

    Mitochondrial protein-lysine N-methyltransferase that trimethylates ATP synthase subunit C, ATP5MC1 and ATP5MC2. Trimethylation is required for proper incorporation of the C subunit into the ATP synthase complex and mitochondrial respiration. Promotes chronic pain. Involved in persistent inflammatory and neuropathic pain: methyltransferase activity in the mitochondria of sensory neurons promotes chronic pain via a pathway that depends on the production of reactive oxygen species (ROS) and on the engagement of spinal cord microglia.

    功能2:

    /

    文献:

    /

    研究领域:

    Others

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    相关实验
    • 利用植物蛋白质芯片研究蛋白磷酸化

      . Y., and Yao, S. Q. (2002) Antibody-based fluorescence detection of kinase activity on a peptide array.  Bioorg.Med. Chem. Lett.  1 2 ,  2085-2088. 7.  Fukunaga, R. and Hunter, T. (1997) M N K 1 , a n e w M A P kinase-activated protein kinase

    • Functional Assays on High‐Content Protein Microarrays

      ?protein interactions, protein?DNA interactions, protein?phospholipid interactions, small?molecule targets, substrates of protein kinases, substrates of ubiquitin E3 ligases, and substrates of methyltransferase. This article provides a detailed illustration

    • 【转帖】[转贴]外源基因在原核细胞中的表达

      基因多个连接,增加稳定表达。 D、酶学和化学法选择性裂解融合蛋白: GrBr.裂解Met,生产胰岛素; Somatomedin、Acromobacter、Protease I(API)特异裂解Lysine,生产d-hANP。 (二) 从表达产物分泌性来分 1、分泌表达:表达载体或外源基因含信号肽。 常用信号肽:b-lactamase、alkaliphospatase、OmpA、ProteinA 优点:1)防止蛋白酶降解2)有利于纯化3)表达产物N端不含有Met 4)大多为可溶性表达

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    ¥9720