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- 文献和实验
- 技术资料
- 保存条件:
We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃.
- 保质期:
Generally, the shelf life of liquid form is 6 months at -20℃/-80℃. The shelf life of lyophilized form is 12 months at -20℃/-80℃.
- 英文名:
Recombinant Human ATP synthase subunit C lysine N-methyltransferase (ATPSCKMT)
- 库存:
200
- 供应商:
武汉华美生物工程有限公司
- 规格:
20μg
英文名称:
Recombinant Human ATP synthase subunit C lysine N-methyltransferase (ATPSCKMT)品名简称:
Recombinant Human ATPSCKMT protein货号:
CSB-CF761288HU规格:
20μg价格:
9720LC-MS/Western Blot:
/SDS-PAGE:
(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.纯度:
Greater than 85% as determined by SDS-PAGE.内毒素:
Not test生物活性:
/基因名:
ATPSCKMTUniprot No.:
Q6P4H8别名:
Protein N-lysine methyltransferase FAM173B;hFAM173B种属:
Homo sapiens (Human)蛋白长度:
Full Length来源:
in vitro E.coli expression system分子量:
32.2 kDa表达区域:
1-233aa氨基酸序列:
MEGGGGIPLETLKEESQSRHVLPASFEVNSLQKSNWGFLLTGLVGGTLVAVYAVATPFVTPALRKVCLPFVPATTKQIENVVKMLRCRRGSLVDIGSGDGRIVIAAAKKGFTAVGYELNPWLVWYSRYRAWREGVHGSAKFYISDLWKVTFSQYSNVVIFGVPQMMLQLEKKLERELEDDARVIACRFPFPHWTPDHVTGEGIDTVWAYDASTFRGREKRPCTSMHFQLPIQA蛋白标签:
N-terminal 10xHis-tagged产品提供形式:
Liquid or Lyophilized powder缓冲液:
If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.储存条件:
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.保质期:
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20℃/-80℃. The shelf life of lyophilized form is 12 months at -20℃/-80℃.货期:
18-23 business days注意事项:
Repeated freezing and thawing is not recommended. Store working aliquots at 4℃ for up to one week.功能1:
Mitochondrial protein-lysine N-methyltransferase that trimethylates ATP synthase subunit C, ATP5MC1 and ATP5MC2. Trimethylation is required for proper incorporation of the C subunit into the ATP synthase complex and mitochondrial respiration. Promotes chronic pain. Involved in persistent inflammatory and neuropathic pain: methyltransferase activity in the mitochondria of sensory neurons promotes chronic pain via a pathway that depends on the production of reactive oxygen species (ROS) and on the engagement of spinal cord microglia.功能2:
/文献:
/研究领域:
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文献和实验. Y., and Yao, S. Q. (2002) Antibody-based fluorescence detection of kinase activity on a peptide array. Bioorg.Med. Chem. Lett. 1 2 , 2085-2088. 7. Fukunaga, R. and Hunter, T. (1997) M N K 1 , a n e w M A P kinase-activated protein kinase
-methyltransferase 9 (PRMT9), partial CSB-YP764718HU SETDB1 Recombinant Human Histone-lysine N-methyltransferase SETDB1 (SETDB1) CSB-EP619960HU TRIM25 Recombinant Human E3 ubiquitin/ISG15 ligase TRIM25 (TRIM25) CSB-MP617909HU 参考
3表达,使间质样细胞只能依赖ELMO2存活;ELMO2缺失导致FAK活性降低、自噬失控和细胞死亡。研究团队通过虚拟筛选获得小分子抑制剂C52,可选择性杀伤间质样肺癌细胞及奥希替尼耐药细胞,对正常细胞无明显毒性,为克服肺癌耐药提供了潜在新策略。 10 靶点:HNMT 应用:为心衰治疗提供了新的干预靶点 来源:Histamine N-methyltransferase upregulation, cardiac hypertrophy
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