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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃.
- 保质期:
Generally, the shelf life of liquid form is 6 months at -20℃/-80℃. The shelf life of lyophilized form is 12 months at -20℃/-80℃.
- 英文名:
Recombinant Human ATP synthase subunit C lysine N-methyltransferase (ATPSCKMT)
- 库存:
200
- 供应商:
武汉华美生物工程有限公司
- 规格:
20μg
英文名称:
Recombinant Human ATP synthase subunit C lysine N-methyltransferase (ATPSCKMT)品名简称:
Recombinant Human ATPSCKMT protein货号:
CSB-CF761288HU规格:
20μg价格:
9720LC-MS/Western Blot:
/SDS-PAGE:
(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.纯度:
Greater than 85% as determined by SDS-PAGE.内毒素:
Not test生物活性:
/基因名:
ATPSCKMTUniprot No.:
Q6P4H8别名:
Protein N-lysine methyltransferase FAM173B;hFAM173B种属:
Homo sapiens (Human)蛋白长度:
Full Length来源:
in vitro E.coli expression system分子量:
32.2 kDa表达区域:
1-233aa氨基酸序列:
MEGGGGIPLETLKEESQSRHVLPASFEVNSLQKSNWGFLLTGLVGGTLVAVYAVATPFVTPALRKVCLPFVPATTKQIENVVKMLRCRRGSLVDIGSGDGRIVIAAAKKGFTAVGYELNPWLVWYSRYRAWREGVHGSAKFYISDLWKVTFSQYSNVVIFGVPQMMLQLEKKLERELEDDARVIACRFPFPHWTPDHVTGEGIDTVWAYDASTFRGREKRPCTSMHFQLPIQA蛋白标签:
N-terminal 10xHis-tagged产品提供形式:
Liquid or Lyophilized powder缓冲液:
If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.储存条件:
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.保质期:
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20℃/-80℃. The shelf life of lyophilized form is 12 months at -20℃/-80℃.货期:
18-23 business days注意事项:
Repeated freezing and thawing is not recommended. Store working aliquots at 4℃ for up to one week.功能1:
Mitochondrial protein-lysine N-methyltransferase that trimethylates ATP synthase subunit C, ATP5MC1 and ATP5MC2. Trimethylation is required for proper incorporation of the C subunit into the ATP synthase complex and mitochondrial respiration. Promotes chronic pain. Involved in persistent inflammatory and neuropathic pain: methyltransferase activity in the mitochondria of sensory neurons promotes chronic pain via a pathway that depends on the production of reactive oxygen species (ROS) and on the engagement of spinal cord microglia.功能2:
/文献:
/研究领域:
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文献和实验. Y., and Yao, S. Q. (2002) Antibody-based fluorescence detection of kinase activity on a peptide array. Bioorg.Med. Chem. Lett. 1 2 , 2085-2088. 7. Fukunaga, R. and Hunter, T. (1997) M N K 1 , a n e w M A P kinase-activated protein kinase
Functional Assays on High‐Content Protein Microarrays
?protein interactions, protein?DNA interactions, protein?phospholipid interactions, small?molecule targets, substrates of protein kinases, substrates of ubiquitin E3 ligases, and substrates of methyltransferase. This article provides a detailed illustration
基因多个连接,增加稳定表达。 D、酶学和化学法选择性裂解融合蛋白: GrBr.裂解Met,生产胰岛素; Somatomedin、Acromobacter、Protease I(API)特异裂解Lysine,生产d-hANP。 (二) 从表达产物分泌性来分 1、分泌表达:表达载体或外源基因含信号肽。 常用信号肽:b-lactamase、alkaliphospatase、OmpA、ProteinA 优点:1)防止蛋白酶降解2)有利于纯化3)表达产物N端不含有Met 4)大多为可溶性表达
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