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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃.
- 保质期:
Generally, the shelf life of liquid form is 6 months at -20℃/-80℃. The shelf life of lyophilized form is 12 months at -20℃/-80℃.
- 英文名:
Recombinant Rat Melanopsin (Opn4)
- 库存:
200
- 供应商:
武汉华美生物工程有限公司
- 规格:
20μg
英文名称:
Recombinant Rat Melanopsin (Opn4)品名简称:
Recombinant Rat Opn4 protein货号:
CSB-CF851271RA规格:
20μg价格:
9720LC-MS/Western Blot:
/SDS-PAGE:
(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.纯度:
Greater than 85% as determined by SDS-PAGE.内毒素:
Not test生物活性:
/基因名:
Opn4Uniprot No.:
Q8R456别名:
(Opsin-4)种属:
Rattus norvegicus (Rat)蛋白长度:
Full Length来源:
in vitro E.coli expression system分子量:
53.9 kDa表达区域:
1-474aa氨基酸序列:
MNSPSESRVPSSLTQDPSFTASPALLQGIWNSTQNISVRVQLLSVSPTTPGLQAAAWVPFPTVDVPDHAHYTLGTVILLVGLTGMLGNLTVIYTFCRNRGLRTPANMLIINLAVSDFLMSFTQAPVFFASSLYKKWLFGETGCKFYAFCGAVFGIVSMITLTAIAMDRYLVITRPLATIGMRSKRRTALVLLGVWLYALAWSLPPFFGWSAYVPEGLLTSCSWDYVTFTPLVRAYTMLLFCFVFFLPLLIIIFCYIFIFRAIRETGRACEGCGESPLRRRQWQRLQSEWKMAKVALIVILLFVLSWAPYSTVALVGFAGYSHILTPYMSSVPAVIAKASAIHNPIIYAITHPKYRAAIAQHLPCLGVLLGVSGQRSHPSLSYRSTHRSTLSSQSSDLSWISGQKRQESLGSESEVGWTDTETTAAWGAAQQASGQSFCSHDLEDGEVKAPSSPQEQKSKTPKTKRHLPSLDRRM蛋白标签:
N-terminal 10xHis-tagged产品提供形式:
Liquid or Lyophilized powder缓冲液:
If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.储存条件:
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.保质期:
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20℃/-80℃. The shelf life of lyophilized form is 12 months at -20℃/-80℃.货期:
3-7 business days注意事项:
Repeated freezing and thawing is not recommended. Store working aliquots at 4℃ for up to one week.功能1:
Photoreceptor that binds cis-retinaldehydes . Contributes to pupillar reflex, photoentrainment and other non-image forming responses to light . May be involved in the optokinetic visual tracking response . May be involved in the regulation of retinal hyaloid vessel growth and regression .功能2:
/文献:
"Phosphorylation of rat melanopsin at Ser-381 and Ser-398 by light/dark and its importance for intrinsically photosensitive ganglion cells (ipRGCs) cellular Ca2+ signaling." Fahrenkrug J., Falktoft B., Georg B., Hannibal J., Kristiansen S.B., Klausen T.K. J Biol Chem 289:35482-35493(2014)研究领域:
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文献和实验起始于视网膜。哺乳动物视网膜中主要存在三类感光细胞:视杆细胞(rods)、视锥细胞(cones)和视网膜自感光神经节细胞(intrinsicallyphotosensitiveretinalganglioncells,ipRGCs)。不同于介导视觉图像编码的经典成像视觉感光细胞(视杆细胞和视锥细胞),ipRGCs 通过其基因 Opn4 编码的感光蛋白视黑素(melanopsin)特异性感知蓝光波段的光,并主要介导非成像视觉功能,如昼夜节律光调节、瞳孔光反射和光调控情绪等。在发育过程中,ipRGCs
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三句话读懂一篇 CNS:昼夜颠倒的睡眠,容易加速肥胖;为什么思考会让人感到疲惫?
本周学术君继续带来 CNS 最新进展,助力大家勇攀科研高峰! 1. Cell:揭示光感知促进脑发育的神经机制 发育早期视觉(光)感知能促进多脑区的协同发育和高级脑功能的形成,但其具体机制不明。 2022 年 8 月 8 日,中国科学技术大学生命科学与医学部薛天教授、鲍进研究员团队在 Cell 杂志发表研究论文 Melanopsin retinal ganglion cells mediate light-promoted brain development。 该研究通过使用离体
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