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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃.
- 保质期:
Generally, the shelf life of liquid form is 6 months at -20℃/-80℃. The shelf life of lyophilized form is 12 months at -20℃/-80℃.
- 英文名:
Recombinant Cowpox virus 14 kDa protein (A27L)
- 库存:
200
- 供应商:
武汉华美生物工程有限公司
- 规格:
20μg
英文名称:
Recombinant Cowpox virus 14 kDa protein (A27L)品名简称:
Recombinant Cowpox virus A27L protein货号:
CSB-MP5087CRF规格:
20μg价格:
1740LC-MS/Western Blot:
/SDS-PAGE:
(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.纯度:
Greater than 95% as determined by SDS-PAGE.内毒素:
Not test生物活性:
/基因名:
A27LUniprot No.:
Q66272别名:
14 kDa protein;14K membrane protein;14kDa fusion protein ;CPXV162 protein ;IMV membrane protein种属:
Cowpox virus (CPV)蛋白长度:
Full Length来源:
Mammalian cell分子量:
14.4 kDa表达区域:
1-110aa氨基酸序列:
MDGTLFPGDDDLAIPATEFFSTKAAKKPEAKREAIVKAEGDDNEETLKQRLTNLEKKITNVTTKFEQIEKCCKRNDEVLFRLENHAETLRAAMISLAKKIDVQTGRRPYE蛋白标签:
C-terminal 10xHis-tagged产品提供形式:
Liquid or Lyophilized powder缓冲液:
If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.储存条件:
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.保质期:
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20℃/-80℃. The shelf life of lyophilized form is 12 months at -20℃/-80℃.货期:
18-28 business days注意事项:
Repeated freezing and thawing is not recommended. Store working aliquots at 4℃ for up to one week.功能1:
/功能2:
/文献:
/研究领域:
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文献和实验Outer Membrane Protein Purification
Class 5 Opa 26–30 kDa Adhesion,opa city protein Opc 25 kDa
Factor Xa Cleavage of MBP-Fusion protein
following ON treatment at RT with 10-30 ug Factor Xa from New England BioLabs per mg fusion protein. For some applications factor Xa (MW 43kDa consisting of two subunits of 27 and 16kDa) must be subsequently removed from the sample by chromatography
Thrombin Cleavage of GST-Fusion protein
to cleave 1 mg of fusion protein at room temperature overnight. 1 X PBS buffer: 140mM NaCl; 2.7mM KCL; 10mM Na2HPO4; 1.8mM KH2PO4; pH 7.3 Thrombin Cleavage of Free Eluted Fusion protein First you have to calibrate amount of thrombin, temperature
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