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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
-20℃ to -80℃
- 保质期:
12个月
- 英文名:
Recombinant Mouse HAI-2 / SPINT2 Protein (His Tag)
- 库存:
99
- 供应商:
北京义翘神州科技股份有限公司
- 规格:
10 µg/20 µg/50 µg
| 规格: | 10 µg | 产品价格: | ¥2310.0 |
|---|---|---|---|
| 规格: | 20 µg | 产品价格: | ¥3870.0 |
| 规格: | 50 µg | 产品价格: | ¥3870.0 |
蛋白名称:Mouse HAI-2 / SPINT2 Protein (His Tag)
蛋白构建:A DNA sequence encoding the extracellular domain (Met 1-Lys 140) of mouse SPINT2 (NP_001076017.1) precursor was expressed with a C-terminal polyhistidine tag.
表达宿主:HEK293 Cells
蛋白纯度:> 90 % as determined by SDS-PAGE
蛋白内毒素:< 1.0 EU per μg of the protein as determined by the LAL method
预测N端:Glu 31
蛋白分子量:The secreted recombinant mouse SPINT2 consists of 122 amino acids and has a calculated molecular mass of 14 kDa. As a result of glycosylation, the recombinant protein migrates as an approximately 22 kDa protein in SDS-PAGE under reducing conditions.
蛋白NP号:NP_001076017.1
蛋白氨基酸序列:Met1-Lys140
蛋白标签:C-His
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文献和实验in the expressed sequence tag or unannotated DNA databases and were novel proteins of unknown function. Since that time, we have been using various molecular biological approaches to determine the functions of some of these proteins
mutations at phenylalanine in a putative PP-1C (the catalytic subunit) binding site motif(K/R)(X)(I/V)XF was unable to rescue Hxk2p dephosphorylation in intact yeast or restore Hxk2p phosphatase activity. These results demonstrate that Reg1p targets PP-1C
标记(peptide tag)的使用,可能会因为空间效应影响蛋白的结合区而使蛋白无法结合;最后,NAPPA的蛋白体外表达系统缺乏后翻译的修饰(posttranslational modification),也会因为无法满足体内蛋白表达的时间和空间的分隔,导致蛋白的折叠和活性的变化。无论如何,NAPPA的出现,给生产蛋白芯片提供了更多的途径,是一个令人鼓舞的重大突破。 Science, Vol 305, Issue 5680, 86-90 , 2 July 2004 Self-Assembling
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