- ￥15570 - 54570
- Sino Biological
- 北京
- 21185-H49H-NA-B
- 2025年08月12日
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- 详细信息
- 文献和实验
- 技术资料
- 保存条件：
-20℃ to -80℃
- 保质期：
12个月
- 英文名：
Recombinant Human CLDN9 -Nanodisc Protein(His & AVI Tag), Biotinylated
- 库存：
99
- 供应商：
北京义翘神州科技股份有限公司
- 规格：
20 µg/100 µg
| 规格： | 20 µg | 产品价格： | ￥15570.0 |
|---|---|---|---|
| 规格： | 100 µg | 产品价格： | ￥54570.0 |
蛋白名称：Human CLDN9 -Nanodisc Protein(His & AVI Tag), Biotinylated
蛋白构建：A DNA sequence encoding the human CLDN9 (O95484) (Met1-Thr184) was expressed, with a polyhistidine tag followed by an AVI tag at the C-terminus. The expressed protein was biotinylated in vivo by the Biotin-Protein ligase (BirA enzyme) which is co-expressed.
Nanodisc is a versatile tool for studying membrane proteins. Using styrene-maleic acid (SMA) copolymer, membrane proteins can be extracted directly from prokaryotic and eukaryotic expression systems in the absence of detergents to preserve the protein structure and function better. Compared to membrane scaffold proteins (MSPs) nanodiscs, SMA nanodiscs also have the advantage of preserving proteins' nature by maintaining native lipids surrounded without introducing any heterologous proteins, which allows studies of protein structure and functions in a native-like environment.
表达宿主：E. coli
蛋白纯度：>80% as determined by SDS-PAGE.
蛋白活性：1.Immobilized Human CLDN9-Nanodisc Protein(His & AVI Tag), Biotinylated (Cat:21185-H49H-NA-B）at 5 μg/mL (100 μL/well) on streptavidin precoated (2 μg/mL，100 μL/well) can bind Anti-Human Claudin9 Antibody, Human IgG1,the EC50 is 10-60 ng/mL. 2.Loaded Biotinylated Recombinant Human CLDN9-Nanodisc Protein, His & AVI Tag(Cat:21185-H49H-NA-B) on SA Biosensor, can bind Anti-CLDN9 antibody with an affinity constant of 11.15 nM as determined in BLI assay (ForteBio Octet Red384) (Routinely tested).
蛋白内毒素：< 1.0 EU per μg protein as determined by the LAL method.
预测N端：Met 1
蛋白分子量：The recombinant human CLDN9 consists of 209 amino acids and has a predicted molecular mass of 22.44 kDa.
蛋白NP号：O95484
蛋白氨基酸序列：Met1-Thr184
蛋白标签：C-His-AVI
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近年来，重组蛋白的应用有了显著增长，与此同时，用于重组蛋白表达和纯化的技术和产品也得以增长。自从亲和标签融合技术出现以来，多种多样的短肽或蛋白亲和标签极大地方便了外源表达重组蛋白的分离与蛋白质复合体的纯化，已成为蛋白质研究领域不可或缺的工具。多聚组氨酸标签（His-tag）是目前高通量蛋白纯化最普遍使用的亲和标签，His 标签融合蛋白的固定化金属离子亲和层析（immobilized metal-ion affinity chromatography, IMAC）被作为主要的蛋白纯化方法。从重组蛋白
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