Sino Biological TIGIT重组蛋白 His Tag his tag蛋白

TIGIT重组蛋白|Recombinant Human TIGIT / VSTM3 Protein (His Tag)（产品说明）
蛋白名称：Human TIGIT / VSTM3 Protein (His Tag)
蛋白构建：A DNA sequence encoding the extracellular domain of human TIGIT (NP_776160.2) (Met22-Pro141) with a C-terminal polyhistidine tag was expressed. This construct has a dimer design.
表达宿主：HEK293 Cells
蛋白纯度：≥ 95 % as determined by SDS-PAGE. ≥ 95 % as determined by SEC-HPLC.
蛋白活性：1.Immobilized Recombinant Human CD155 / PVR / NECL5 Protein (Fc Tag) (Cat:10109-H02H) at 2μg/mL (100μL/well) can bind Recombinant Human TIGIT / VSTM3 Protein (His Tag) (Cat:10917-H08H3), the EC50 is 6-18 ng/mL. 2.Loaded Recombinant Human CD155/PVR Protein, hFc Tag (Cat. No. 10109-H02H) on ProA Biosensor, can bind Recombinant Human TIGIT Protein, His Tag (Cat. No. 10917-H08H3) with an affinity constant of 0.51 nM as determined in BLI assay (Sartorius Octet RED384) (Routinely tested). 3.Recombinant Human CD155/PVR Protein, hFc Tag (Cat. No. 10109-H02H) captured on Protein A chip can bind Recombinant Human TIGIT Protein(Dimer), His Tag (Cat. No. 10917-H08H3) with an affinity constant of 3.28 nM as determined in an SPR assay (Biacore 8K) (Routinely tested).
蛋白内毒素：< 1.0 EU per μg protein as determined by the LAL method.
预测N端：Met 22
蛋白分子量：The recombinant human TIGIT predicts a molecular mass of 28.81 kDa. It migrates as an approximately 36.72 kDa band in SDS-PAGE under reducing conditions.
蛋白NP号：NP_776160.2
蛋白氨基酸序列：Met22-Pro141
蛋白标签：C-His